Publications by authors named "Tomoko Ohtsuka"

We had experienced 117 Japanese Fabry patients (72 males and 45 females) from 1977 to 2006, and then we generated an improved Fabry analysis system in 2007 and have found 196 ones (95 males and 101 females) since then. In this study, we summarized the data of the patients and tried to elucidate the molecular and biochemical characteristics of Japanese Fabry patients. Gene analysis revealed various mutations, including missense mutations (56.

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All novel proteins must be assessed for their potential allergenicity before they are introduced into the food market. One method to achieve this is the 2001 FAO/WHO Decision Tree recommended for evaluation of proteins from genetically modified organisms (GMOs). It was the aim of this study to investigate the allergenicity of microbial transglutaminase (m-TG) from Streptoverticillium mobaraense.

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We have held practical classes called chozaigijutsu-konwakai(Konwakai) for pharmacists working in drugstores every month since December 1994. Konwakai is composed of lectures and practice. The practice is carried out for pharmacists to obtain a better understanding of prescriptions and compounding them in exercises.

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Article Synopsis
  • Researchers studied how to efficiently refold denatured microbial transglutaminase (MTG) using different buffers after it was denatured with urea.
  • Low protein recovery was observed with neutral and alkaline buffers, but using mildly acidic buffers resulted in better recovery with partial enzymatic activity.
  • Further experiments showed that adjusting the pH to 6 after 2 hours of incubation at pH 4 improved the enzyme's activity to nearly that of the native form, highlighting the importance of temperature and pH in the refolding process.
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