Establishment of a simple one-step method for oligodendrocyte progenitor cell preparation from rodent brains.

Background: Oligodendrocytes, which form myelin, enable rapid and efficient nerve conduction. Destruction of myelin causes demyelinating diseases such as multiple sclerosis. Primary oligodendrocyte progenitor cells (OPCs) from postnatal rodents have been utilized to elucidate the developmental mechanism of oligodendrocytes in vitro.

Ultrafilter co-culture, a new method for estimating the molecular mass of bioactive substances, indicates a small molecule neurotrophic substance is released from cultured cerebellar granule neurons of the BALB/c mouse.

Cultured rat cerebellar granule neurons (CGNs), which require a depolarizing agent in the medium for long-term survival, are widely used for the analysis of mechanisms underlying the activity-dependent survival of neurons. It was recently found that this is not the case for BALB/c mouse CGNs, which survive without a depolarizing agent. Co-culture experiments indicated that the mouse cells release a neurotrophic substance.