Transduction of MyoD protein into myoblasts induces myogenic differentiation without addition of protein transduction domain.

Protein transduction is a new technology with the potential for controlling cellular functions. Addition of tissue-specific transcription factors into cells could induce a specific differentiation pathway. In this paper, MyoD protein, a muscle-specific transcription factor, was introduced into the mouse myoblast cell line C2C12 to induce myogenic differentiation.

Transduction of NeuroD2 protein induced neural cell differentiation.

NeuroD2, one of the neurospecific basic helix-loop-helix transcription factors, has the ability to induce neural differentiation in undifferentiated cells. In this paper, we show that transduction of NeuroD2 protein induced mouse neuroblastoma cell line N1E-115 into neural differentiation. NeuroD2 has two basic-rich domains, one is nuclear localization signal (NLS) and the other is basic region of basic helix-loop-helix (basic).

Classification of DNA-binding mode of antitumor and antiviral agents by the electrochemiluminescence of ruthenium complex.

The DNA-binding mode of antitumor and antiviral agents has been evaluated by electrochemiluminescence (ECL) of tris(1,10-phenanthroline)-ruthenium complex (Ru(phen)(3)(2+)) in the presence of oxalate ion in pH 7.3 Tris buffer solution. An emission of Ru(phen)(3)(2+) was observed repeatedly with a voltage above 1000mV subjected to a potential sweep from 0 to 1250mV.