Solvent-assisted mechanochemical crystallization of the metal-free perovskite solid solution (Hdabco, Hhmta)NH(BF).

All-proportional solid solutions of the metal-free perovskite (Hdabco, Hhmta)(NH)(BF) ((d,h)-BF) were crystallized a mechanochemical method. Their molecular dynamics depend on the ratio with a compositional boundary at = 0.43, where Hdabco was deduced to be at a dynamic disorder state, even below phase transition temperature to a plastic crystalline phase seen at = 0.

Alzheimer's disease-related protein hGas7b interferes with kinesin motility.

In the previous study, we reported the important properties of hGas7b (i) that binds to phospho-tau and facilitates microtubule polymerization and (ii) the level of hGas7b is very low in the brains of patients with Alzheimer's disease. These results led us to study the function of hGas7b in detail. We focused on the effect of hGas7b on microtubule dynamics in the absence of tau, on the assumption of healthy tau decrease in the brains of Alzheimer's disease.

A novel role for hGas7b in microtubular maintenance: possible implication in tau-associated pathology in Alzheimer disease.

Here, we report a novel role for hGas7b (human growth arrest specific protein 7b) in the regulation of microtubules. Using a bioinformatic approach, we studied the actin-binding protein hGas7b with a structural similarity to the WW domain of a peptidyl prolyl cis/trans isomerase, Pin1, that facilitates microtubule assembly. Thus, we have demonstrated that hGas7b binds Tau at the WW motif and that the hGas7b/Tau protein complex interacts with the microtubules, promoting tubulin polymerization.

Direct optical microscopic observation of the microtubule polymerization intermediate sheet structure in the presence of gas7.

The process of microtubule elongation is thought to consist of two stages-formation of a tubulin sheet structure and its closure into a tube. However, real-time observation of this process has been difficult. Here, by utilizing phospho-tau binding protein Gas7 (growth-arrest-specific protein 7), we visualized the polymer transformation process by dark-field microscopy.