GIP1 and GIP2 Contribute to the Maintenance of Genome Stability at the Nuclear Periphery.

The maintenance of genetic information is important in eukaryotes notably through mechanisms occurring at the nuclear periphery where inner nuclear membrane proteins and nuclear pore-associated components are key factors regulating the DNA damage response (DDR). However, this aspect of DDR regulation is still poorly documented in plants. We addressed here how genomic stability is impaired in the () double mutants showing defective nuclear shaping.

Chromatin Manipulation and Editing: Challenges, New Technologies and Their Use in Plants.

An ongoing challenge in functional epigenomics is to develop tools for precise manipulation of epigenetic marks. These tools would allow moving from correlation-based to causal-based findings, a necessary step to reach conclusions on mechanistic principles. In this review, we describe and discuss the advantages and limits of tools and technologies developed to impact epigenetic marks, and which could be employed to study their direct effect on nuclear and chromatin structure, on transcription, and their further genuine role in plant cell fate and development.

Serum S100B protein in early management of patients after mild traumatic brain injury.

Background And Purpose: Neuronal protein S100B assays are available now with a perspective of being an early screening tool for serious intracranial injury. The aim of the study was to correlate early S100B measurements and initial CCT findings in the patients sustaining mild traumatic brain injury (MTBI).

Methods: The prospective study included patients of all ages with a history of MTBI.

[CT findings in alcohol intoxicated patients after mild brain injury].

Objective: To find out severity of brain injury in alcohol intoxicated patients after mild (GCS 13-15) brain injury.

Methods: A retrospective study of all patients older than 15 years in whom cranial CT (CCT) scan was performed between October 2006 and September 2007. Information of alcohol intoxication, injury mechanism, details of facial/head injuries, admission GCS, CCT findings and type of treatment were retrieved from patients' medical records.

[Renal excretion of amino acids in patients on a regular dialysis program].

In 10 patients with chronic renal failure and a polyfructosan S clearance (CPFS): 1.91-12.70 ml/min (mean 5.

Renal amino acid excretion and aging.

The urinary excretion and serum concentration of amino acids were studied in 62 healthy individuals aged 15 to 70 years. In elderly subjects (61-70 years), it was found that renal amino acid clearance per 100 ml GFR (fractional excretion, FE) rose significantly in the following amino acids: CYS, VAL, MET, ILE and LEU. Since the serum concentrations of these amino acids showed no significant changes, but the GFR was reduced, it can be concluded that the raised FE of these amino acids was due to a decrease in their effective tubular reabsorption.

Optimal amino acid composition of diets for patients with chronic renal failure.

When following the spectrum of plasmatic amino acids in patients with chronic renal failure in course of a six-week treatment with low-protein diets, an increase of non-essential amino acids, glycine and alanine, was observed in the case of a diet containing 20 g of proteins. A further decrease of tyrosine and threonine, which were decreased already before the therapy, suggests the presence of an effect of the reduced intake of proteins on the levels of these amino acids. High levels of methionine occurring in the case of the diet containing 20 g of proteins a day, supplemented by methionine to a minimally necessary requirement according to Rose, show different requirements) for this amino acid in patients with the chronic renal failure.

[Effect of furosemide on the amino acid content of serum and elimination by the kidneys].

The effect of intraventously administered furosemide (20 mg) on the concentration of 21 amino acids in the blood serum and urine of 6 healthy volunteers was investigated. A substantially increased concentration of glutaminic acid and citrulline and a statistically significantly greater excretion of arginine with urine were ascretained.

Amino acid metabolism in the heart muscle in subjects with ischaemic heart disease at rest and during pacing.

In nine patients with ischaemic heart disease at rest and during pacing differences of free plasma amino acids, lactate, ammonia and uric acid between arterial blood and blood in the coronary sinus (a-cs differences) were investigated. At rest one single significant difference was found, i.e.

Renal clearance of amino acids in patients with severe chronic renal failure.

The renal clearance of 21 amino acids was investigated in 5 patients with chronic renal failure (inulin clearance = 5--10ml/min) during the 6-week administration of a low-protein diet. The results were compared with values obtained in 6 volunteers. The renal clearance of all investigated amino acids (with the exception of Asp, His, Tau) did not differ significantly from healthy controls.

Changes of free amino acids in plasma of healthy subjects induced by physical exercise.

In eight healthy men a 20-min load of 1.5 W/kg body weight on a bicycle ergometer led to a significant increase of alanine and decline of leucine. Exhausting exercise caused in the same subjects a highly significant increase of alanine and decline of isoleucine, threonine, ornithine, leucine, serine, glycine, and asparasine and glutamine.