Publications by authors named "Tomer Schvartz"
Article Synopsis
- Genetic code expansion allows for the addition of non-canonical amino acids (ncAAs) into proteins by using modified tRNA systems that recognize stop codons.
- The study found that reducing the number of tRNA genes (PylT) did not affect protein expression in COS7 cells, while enhancing imaging quality by lowering background noise in live-cell imaging.
- These findings suggest that the optimal number of PylT genes can be adjusted based on the specific cell line, type of ncAA, and intended application in experiments.
Genetic code expansion and bioorthogonal labeling provide for the first time a way for direct, site-specific labeling of proteins with fluorescent-dyes in live cells. Although the small size and superb photophysical parameters of fluorescent-dyes offer unique advantages for high-resolution microscopy, this approach has yet to be embraced as a tool in live cell imaging. Here we evaluated the feasibility of this approach by applying it for α-tubulin labeling.
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