[Problems of prevention and control of poliomyelitis in Poland].

In the fifties and the sixties of 20th century big epidemic and increasing numbers of poliomyelitis cases were observed in Poland as well in other countries of Europe and world. Inactivated Salk's vaccine in injections as well as oral attenuated vaccine were introduced to the control and prevention of this disease. As the result of it poliomyelitis was eradicated in American, European and of West Pacific Region of WHO.

Telomerase activity and serum levels of p53 protein as prognostic factors of survival in patients with advanced non-small cell lung cancer.

Purpose: Evaluation of relationships between survival time of patients with advanced, non-resectable non-small cell lung cancer (NSCLC) and telomerase activity in aspirates, collected from primary lung tumours, and serum p53 protein levels.

Material And Methods: The study group consisted of 52 patients with advanced (stage IIIB and IV) non-small cell lung cancer. In all of them, transthoracic fine-needle biopsy (TFNB) of focal pulmonary lesion was performed.

Telomerase activity in transthoracic fine-needle biopsy aspirates from non-small cell lung cancer as prognostic factor of patients' survival.

Purpose: Evaluation of the relationship between telomerase activity in transthoracic fine-needle biopsy (TFNB) aspirates collected from peripheral non-small cell lung cancer (NSCLC), cancer advancement, risk of death and free of cancer recurrence survival.

Material And Methods: The study group consisted of 93 patients with peripheral infiltration of the lung. All of them had TFNB of the focal pulmonary lesion performed.

[An evaluation of the occurrence of pneumocystis infection through examination of pharyngeal and oral swabs using the nested PCR method].

A method of collecting pharyngeal and oral swabs from humans and laboratory rats, to be examined later for Pneumocystis infection with simple and nested PCR, was developed. The swabs were obtained from 15 HIV-infected patients, including 5 suffering from PCP, and from 10 immunocompetent children aged 2 to 6 years. Furthermore, the swabs were taken from 30 healthy laboratory rats and 23 animals subjected to immunosuppressive treatment.

Three dimensional model of severe acute respiratory syndrome coronavirus helicase ATPase catalytic domain and molecular design of severe acute respiratory syndrome coronavirus helicase inhibitors.

The modeling of the severe acute respiratory syndrome coronavirus helicase ATPase catalytic domain was performed using the protein structure prediction Meta Server and the 3D Jury method for model selection, which resulted in the identification of 1JPR, 1UAA and 1W36 PDB structures as suitable templates for creating a full atom 3D model. This model was further utilized to design small molecules that are expected to block an ATPase catalytic pocket thus inhibit the enzymatic activity. Binding sites for various functional groups were identified in a series of molecular dynamics calculation.

[Influence of nicotine addiction on telomerase activity in malignant non-small cell lung tumors].

Telomerase synthesizes telomeric DNA repeats at the ends of eukaryotic chromosomes and inhibits the natural senescence of different cells. Its increased activity in malignant tumors is considered to be an independent prognostic factor of the disease course. Aim of the study was valuation of influence of prolonged tobacco smoking on telomerase expression in non-small cell lung cancer (NSCLC).