Development of microsatellite markers for the perennial plant .

Premise: Polymorphic microsatellite markers were developed to study genetic diversity and genetic structure of populations of the locally endangered species (Tofieldiaceae).

Methods And Results: Nineteen polymorphic microsatellite loci were developed using DNA-enriched libraries sequenced by Ilumina technology and were then used to genotype 101 individuals from five populations from Austria, Slovakia, Poland, and the Czech Republic. Of the markers tested, 68% were polymorphic in four of the five investigated populations, with one marker polymorphic in all populations.