Assessing the Heat Tolerance of Meiosis in Spanish Landraces of Tetraploid Wheat .

Heat stress alters the number and distribution of meiotic crossovers in wild and cultivated plant species. Hence, global warming may have a negative impact on meiosis, fertility, and crop productions. Assessment of germplasm collections to identify heat-tolerant genotypes is a priority for future crop improvement.

Genomic and Meiotic Changes Accompanying Polyploidization.

Hybridization and polyploidy have been considered as significant evolutionary forces in adaptation and speciation, especially among plants. Interspecific gene flow generates novel genetic variants adaptable to different environments, but it is also a gene introgression mechanism in crops to increase their agronomical yield. An estimate of 9% of interspecific hybridization has been reported although the frequency varies among taxa.

Analytical Methodology of Meiosis in Autopolyploid and Allopolyploid Plants.

Meiosis is the cellular process responsible for producing gametes with half the genetic content of the parent cells. Integral parts of the process in most diploid organisms include the recognition, pairing, synapsis, and recombination of homologous chromosomes, which are prerequisites for balanced segregation of half-bivalents during meiosis I. In polyploids, the presence of more than two sets of chromosomes adds to the basic meiotic program of their diploid progenitors the possibility of interactions between more than two chromosomes and the formation of multivalents, which has implications on chromosome segregations and fertility.

Variable Patterning of Chromatin Remodeling, Telomere Positioning, Synapsis, and Chiasma Formation of Individual Rye Chromosomes in Meiosis of Wheat-Rye Additions.

Meiosis, the type of cell division that halves the chromosome number, shows a considerable degree of diversity among species. Unraveling molecular mechanisms of the meiotic machinery has been mainly based on meiotic mutants, where the effects of a change were assessed on chromosomes of the particular species. An alternative approach is to study the meiotic behavior of the chromosomes introgressed into different genetic backgrounds.

Contribution of Structural Chromosome Mutants to the Study of Meiosis in Plants.

Dissection of the molecular mechanisms underlying the transition through the complex events of the meiotic process requires the use of gene mutants or RNAi-mediated gene silencing. A considerable number of meiotic mutants have been isolated in plant species such as Arabidopsis thaliana, maize or rice. However, structural chromosome mutants are also important for the identification of the role developed by different chromosome domains in the meiotic process.

Forcing the shift of the crossover site to proximal regions in wheat chromosomes.

Terminal deletions obligate the first crossover to be formed in more proximal positions. This increases the recombination rate in intercalary intervals but not in the proximity of the centromere. Crossovers are not uniformly distributed along chromosomes in wheat.

Dynamics of rye telomeres in a wheat background during early meiosis.

Migration of the telomere of the short arm of rye chromosome 5R (5RS) during bouquet organization is dependent on the conformation that this chromosome adopts in its intact, submetacentric, or truncated, metacentric, form. In order to establish whether the telomere migration dependence on chromosome conformation is a common feature of all rye chromosomes, the behavior of the telomeres of 2 other rye chromosomes, 1R and 6R, with apparent differences in the arm ratio, has been studied at the bouquet stage and compared with that of 5R. The presence of subtelomeric heterochromatic chromomeres in both arms of 1R and 6R, which were visualized by FISH, revealed the position of the adjacent telomeres in the bouquet.

Identifying crossover-rich regions and their effect on meiotic homologous interactions by partitioning chromosome arms of wheat and rye.

Chiasmata are usually formed in the distal half of cereal chromosomes. Previous studies showed that the crossover-rich region displays a more active role in homologous recognition at early meiosis than crossover-poor regions in the long arm of rye chromosome 1R, but not in the long arm of chromosome 5R. In order to determine what happens in other chromosomes of rye and wheat, we have partitioned, by wheat-rye translocations of variable-size, the distal fourth part of chromosome arms 1BS and 2BL of wheat and 1RS and 2RL of rye.

Dynamics of rye chromosome 1R regions with high or low crossover frequency in homology search and synapsis development.

In many organisms, homologous pairing and synapsis depend on the meiotic recombination machinery that repairs double-strand DNA breaks (DSBs) produced at the onset of meiosis. The culmination of recombination via crossover gives rise to chiasmata, which locate distally in many plant species such as rye, Secale cereale. Although, synapsis initiates close to the chromosome ends, a direct effect of regions with high crossover frequency on partner identification and synapsis initiation has not been demonstrated.

Finding the correct partner: the meiotic courtship.

Homologous chromosomes are usually separated at the entrance of meiosis; how they become paired is one of the outstanding mysteries of the meiotic process. Reduction of spacing between homologues makes possible the occurrence of chromosomal interactions leading to homology detection and the formation of bivalents. In many organisms, telomere-led chromosome movements are generated that bring homologues together.

Genetic variation in natural populations of Stipa tenacissima from Algeria.

Intermicrosatellite PCR [inter-simple sequence repeat (ISSR)-PCR] markers and cytogenetics criteria were used to assess the level of genetic diversity and genetic structure in 17 populations of Stipa tenacissima (Gramineae) from Algeria. All populations sampled in the steppe area were diploids (2n = 2x = 24), and those sampled in the dry area were hexaploids (2n = 6x = 72). The dendrogram based on ISSR-PCR showed homogeneity within populations and large variability among populations.

Terminal regions of wheat chromosomes select their pairing partners in meiosis.

Many plant species, including important crops like wheat, are polyploids that carry more than two sets of genetically related chromosomes capable of meiotic pairing. To safeguard a diploid-like behavior at meiosis, many polyploids evolved genetic loci that suppress incorrect pairing and recombination of homeologues. The Ph1 locus in wheat was proposed to ensure homologous pairing by controlling the specificity of centromere associations that precede chromosome pairing.

Effect of colchicine and telocentric chromosome conformation on centromere and telomere dynamics at meiotic prophase I in wheat-rye additions.

Association of telomeres in a bouquet and clustering of centromere regions have been proposed to be involved in the search and recognition of homologous partners. We have analysed the role of these structures in meiotic chromosome pairing in wheat-rye addition lines by applying colchicine for disturbing presynaptic telomere movements and by modifying the centromere position from submetacentric to telocentric for studying centromere effects. Rye chromosomes, wheat and rye centromeres, and telomeres were identified by fluorescence in-situ hybridization.

Chromosome arrangement and behaviour of two rye homologous telosomes at the onset of meiosis in disomic wheat-5RL addition lines with and without the Ph1 locus.

Fluorescence in-situ hybridization (FISH) of total genomic and repetitive DNA on microsporocytes of ditelocentric addition lines of rye 5RL in hexaploid wheat was performed to study the behaviour of the rye homologous chromosome arms in relation to centromere and telomere dynamics at premeiotic interphase and meiotic prophase I. By comparing isogenic lines with and without the Ph1 locus, we established the effect of the Ph1 gene on appearance and behaviour of the rye chromosomes. Ph1 and ph1b lines demonstrated similar premeiotic chromosome arrangement with the two rye homologues occupying separated domains despite the occurrence of centromere association.

Prolymphocytic leukaemia and Hodgkin's lymphoma.

We describe a case of B-prolymphocytic leukaemia (B-PLL) who, following a long-lasting remission with fluradabine, developed a Hodgkin's lymphoma (HL) with bone marrow involvement. A 75-yr-old male was found to have a lymphocytosis [white blood cell (WBC) count = 146 x 10(9) L(-1)], small volume axillary lymphadenopathy, and hepatosplenomegaly. The majority of circulating lymphocytes had a round nucleus and prominent single nucleolus.