[A case of ectopic breast tissue located in the axilla].

Authors have presented a very rare case of young woman favourable surgically treated for morphologically correct breast tissue in the unilateral axilla. They have paid the peculiar attention to possibility of ectopically placed breast tissue malignancy of every that case.

[Immunoenzyme (ELISA) diagnosis of spring viremia in carp].

The enzymoimmunologic (ELISA) method was used to diagnose spring viremia in carp. The specific antibodies to Rhabdovirus carpio in the fish sera were detected by this method. Reaction specificity was tested by the use of viral and control antigens, by examination of positive and negative sera and by serum-free control incubation.

[Isolation and identification of the infectious pancreatic necrosis virus in trout].

The first isolation and identification of the infectious pancreatic necrosis virus in trout in Czechoslovakia is described. The RTG-2, PG and CHSE cell lines were used for isolation and the identification was made by the methods of electron microscopy, cross virus-neutralizing test, and immunofluorescence. As demonstrated, all the isolates in the Czechoslovak territory are serologically identical and are closely related (or perhaps congruent) with the Sp reference strain which has the highest frequency in Europe.

[Diagnosis of infectious pancreatic necrosis (IPN) of trout using immunofluorescence].

Until recently, demonstration of infectious pancreatic necrosis was conditioned by the isolation of virus. By means of the immunofluorescence method it is possible to make a rapid and accurate diagnosis, as well as to determine the development of infection. In acute cases, the virus in fry was demonstrated mainly in the cytoplasm of epithelial cells of guts; in Pd1-2 trout, the virus was proved in the cytoplasm of epithelial cells of primary kidney tubules and in the cytoplasm of epithelial cells of pancreatic acini.

[Necrotic processes in the gill tissue of carp caused by changes in the aquatic environment].

Histological findings show that various pathogenic processes are involved in the cases of clinically detected branchionecrosis. The changes in gills occurring at toxic branchionecrosis are a necrobiotic process in their nature; in gill lamellae this process manifests itself as a swelling of the respiratory epithelium and as dilation of lamellar sinuses, and in stratified epithelium of gill arch and filaments it takes on the form of the hypertrophy and hyperplasia of epithelial cells. In severe cases the swollen epithelium forms deposits filling up also the interlamellar space.

[Gill structure in healthy carp].

When the gills of carp are studied, this organ should be evaluated from there planes of projection: sagittal, transversal and horizontal. Lamellae, the functional base of the gills, provide the exchange of oxygen between blood and water. Lamellae consist of a network of sinuses with a skeleton formed by columnar cells and with walls constituted by the cytoplasmic processes of these cells.

[Residual larval effects of permethrin and resistance in the housefly].

The effectiveness of synthetic pyrethroid permethrin in a special preparation Coopex, concentrations 0.25 and 0.5% against larval stages of housefly (Musca domestica) was tested.