Publications by authors named "Tom W Bargar"

Enterohemorrhagic (EHEC), a pathogenic subset of Shiga toxin-producing (STEC), is an important cause of hemorrhagic colitis and hemolytic-uremic syndrome (HUS), and a rare cause of urinary tract infections (UTIs) with associated HUS. EHEC strains attach intimately to intestinal epithelium with formation of actin pedestals (attaching-effacing (A/E) lesions); however, the mechanism of EHEC attachment to the uroepithelium is unknown. We conducted a retrospective study on archived urinary bladder specimens from gnotobiotic piglets that naturally developed cystitis associated with EHEC O157:H7 infection following oral inoculation and fecal shedding.

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Fibroblasts are heterogeneous mesenchymal cells that play important roles in the production and maintenance of extracellular matrix. Although their heterogeneity is recognized, progenitor progeny relationships among fibroblasts and the factors that control fibroblast differentiation are poorly defined. The current study was designed to develop a reliable method that would permit in vitro differentiation of fibroblast-like cells from human and murine embryonic stem cells (ESCs).

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Asthmatic airway remodeling is characterized by goblet cell hyperplasia, angiogenesis, smooth muscle hypertrophy, and subepithelial fibrosis. This study evaluated whether acquired changes in fibroblast phenotype could contribute to this remodeling. Airway and parenchymal fibroblasts from control or chronically ovalbumin (OVA)-sensitized and challenged "asthmatic" mice were assessed for several functions related to repair and remodeling +/- exogenous transforming growth factor (TGF)-beta.

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Pituitary pars nervosa-pars intermedia of Anolis carolinensis, Rana pipiens and Hyla crysoscelis were perifused with synthetic medium 199 for up to 35 h. The pre- and post-perifused tissues were examined by electron microscopy. No neuronal endings were found in Anolis tissue, but both Rana and Hyla had occasional synaptic end bulbs, which remained visible in the post-perifused tissue, although the synaptic vesicles appeared to cluster in the center of the end bulbs.

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