Publications by authors named "Tom Michaels"

Article Synopsis
  • Hemp is being explored as a sustainable source of plant protein, and this study evaluates two methods for extracting high-purity hemp protein isolates: alkaline extraction with isoelectric precipitation (AE-IEP) and salt extraction with ultrafiltration (SE-UF).
  • Both extraction methods achieved high protein purity (86.6-88.1%) and yields (81.6-87.3%), although the isolates showed poor solubility at neutral pH compared to soy and pea proteins, although they performed better in acidic conditions.
  • The findings indicate that while there are structural and functional differences between the two extraction methods, the overall protein characteristics can potentially inform future extraction improvements and hemp breeding efforts for better food applications.
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Breeding a model plant that encompasses individual traits thought to enhance yield potential, known as ideotype breeding, has traditionally focused on phenotypic selection of plants with desirable morphological traits. Broadening this breeding method to the molecular level through the use of molecular markers would avoid the environmental interactions associated with phenotypic selection. A population of 110 F5 recombinant inbred lines (RILs), derived from the cross between WO3391 and 'OAC Speedvale', was used to develop a genetic linkage map consisting of 105 random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), and sequence-tagged site (STS) markers.

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Recovery of superior individuals from a cross based solely on the phenotypic characteristics ofsingle-plant selections is inefficient because some traits, like yield, have low heritabilities, or because it is difficult to create the correct conditions for selection, as with disease resistance. In contrast, molecular markers are highly heritable and unaffected by environmental conditions. The objective of this study was to investigate the potential of molecular markers to identify superior lines in a breeding population by examining relationships between genetic distances (GDs) and phenotypic data for eight agronomic and architectural traits (branch angle, height, hypocotyl diameter, lodging, maturity, upper pods, pods per plant, and yield) obtained from three locations over a two-year period.

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