Publications by authors named "Tom Kazimiers"

Article Synopsis
  • The research focuses on the fruit fly Drosophila melanogaster as a crucial model in neuroscience, aided by extensive resources like the FlyWire whole-brain connectome and a hierarchical annotation of neuron classes and types.
  • The study reveals 8,453 annotated cell types, with 4,581 being newly identified, highlighting the complexity of the fly brain and emphasizing the difficulty in reidentifying some hemibrain cell types in FlyWire.
  • A new definition of cell type is proposed based on cell similarities across different brains, and the study illustrates findings related to neuron connectivity, structural stability, and a consensus atlas for the fly brain's neuroanatomy, supporting future comparative studies.
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Article Synopsis
  • The fruit fly is a key model organism in neuroscience due to its complex behaviors and accessible nervous system, bolstered by collaborative genetic resources.*
  • The FlyWire project has produced the first complete brain connectome of an adult fruit fly, providing a detailed catalog of approximately 130,000 neurons, including 4,552 cell types.*
  • Analysis indicated that while some neuronal connections were stable, others showed variability across individuals, revealing complexities in brain function and suggesting some cell types from previous studies may not be reliably identified in this new dataset.*
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Brains contain networks of interconnected neurons and so knowing the network architecture is essential for understanding brain function. We therefore mapped the synaptic-resolution connectome of an entire insect brain ( larva) with rich behavior, including learning, value computation, and action selection, comprising 3016 neurons and 548,000 synapses. We characterized neuron types, hubs, feedforward and feedback pathways, as well as cross-hemisphere and brain-nerve cord interactions.

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Cells form networks in animal tissues through synaptic, chemical, and adhesive links. Invertebrate muscle cells often connect to other cells through desmosomes, adhesive junctions anchored by intermediate filaments. To study desmosomal networks, we skeletonised 853 muscle cells and their desmosomal partners in volume electron microscopy data covering an entire larva of the annelid .

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Associative memory formation and recall in the fruit fly Drosophila melanogaster is subserved by the mushroom body (MB). Upon arrival in the MB, sensory information undergoes a profound transformation from broadly tuned and stereotyped odorant responses in the olfactory projection neuron (PN) layer to narrowly tuned and nonstereotyped responses in the Kenyon cells (KCs). Theory and experiment suggest that this transformation is implemented by random connectivity between KCs and PNs.

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We develop an automatic method for synaptic partner identification in insect brains and use it to predict synaptic partners in a whole-brain electron microscopy dataset of the fruit fly. The predictions can be used to infer a connectivity graph with high accuracy, thus allowing fast identification of neural pathways. To facilitate circuit reconstruction using our results, we develop CIRCUITMAP, a user interface add-on for the circuit annotation tool CATMAID.

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Neuronal circuit mapping using electron microscopy demands laborious proofreading or reconciliation of multiple independent reconstructions. Here, we describe new methods to apply quantitative arbor and network context to iteratively proofread and reconstruct circuits and create anatomically enriched wiring diagrams. We measured the morphological underpinnings of connectivity in new and existing reconstructions of Drosophila sensorimotor (larva) and visual (adult) systems.

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Membrane trafficking is key to the cell biological mechanisms underlying development. Rab GTPases control specific membrane compartments, from core secretory and endocytic machinery to less-well-understood compartments. We tagged all 27 Drosophila Rabs with YFP(MYC) at their endogenous chromosomal loci, determined their expression and subcellular localization in six tissues comprising 23 cell types, and provide this data in an annotated, searchable image database.

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