Publications by authors named "Tom Hooper"
Turkey coronavirus (TCoV) infection causes acute atrophic enteritis in the turkey poults, leading to significant economic loss in the U.S. turkey industry.
View Article and Find Full Text PDFThis study reports on the development of a reverse transcriptase-polymerase chain reaction (RT-PCR) for the specific detection of turkey coronavirus (TCoV). Of the several sets of primers tested, 1 set of primers derived from the P gene and 2 sets derived from the N gene of TCoV could amplify the TCoV genome in the infected samples. The RT-PCR was sensitive and specific for TCoV and did not amplify other avian RNA and DNA viruses tested except the infectious bronchitis virus (IBV).
View Article and Find Full Text PDFArticle Synopsis
- The study aimed to assess the antigenicity of turkey coronavirus (TCV) isolates from various locations and their interactions with antibodies to different viruses.
- Seventeen TCV isolates were collected from turkey farms and tested against various antibodies, showing similar reactivity patterns among all isolates and specific reactions to infectious bronchitis virus (IBV) antibodies.
- The findings reveal that the TCV isolates are antigenically similar and closely related to IBV, with a notable exception of a single case indicating potential coinfection with enterovirus.
The effect of immunosuppression on protective immunity of turkey poults against infection with turkey coronavirus.
Comp Immunol Microbiol Infect Dis
March 2002
The objective of the present study was to evaluate the protective effect of humoral and cellular immunities on turkeys infected with turkey coronavirus (TCV). Two trials were conducted with two separate hatches of turkey poults. Turkey's were experimentally immunosuppressed with cyclosporin A (CsA) or cyclophosphamide (CY) and infected with TCV.
View Article and Find Full Text PDFArticle Synopsis
- The study aimed to investigate a turkey coronavirus linked to enteritis and high mortality rates in turkey poults.
- Researchers identified the virus through various methods, including electron microscopy and immunofluorescent assays, and characterized its properties using sucrose density gradient ultracentrifugation, revealing distinct buoyant densities and spherical-shaped particles.
- Sequence analysis showed a significant similarity between the turkey coronavirus spike protein gene and those of human and bovine coronaviruses, confirming its association with the observed disease in turkey poults.