In vivo use of human fibrin glue under the subperiosteal and subcutaneous planes in Holtzman rats.

Background: Commercial fibrin sealant has been increasingly used off-label in the United States for aesthetic surgical procedures to minimize postoperative drainage, hematoma and seroma collections, ecchymosis, and edema.

Objective: We sought to determine the optimal concentrations of thrombin and fibrinogen to extend the sealant adhesion time and to maintain the maximal glue strength, respectively, in subperiosteal and subcutaneous planes in Holtzman rats.

Methods: Three preparations of 2-component fibrin sealant from the Hemaseel APR kit (Haemacure Corp.