Publications by authors named "Toby J Zaleski"
J Am Chem Soc
December 2005
Article Synopsis
- Picromycin/methymycin synthase (PICS) is a modular polyketide synthase involved in creating two key compounds, 10-deoxymethynolide and narbonolide, which serve as precursors for antibiotics methymycin and picromycin.
- The study focuses on PICS module 2, which functions as a dehydratase and was expressed in E. coli to analyze its ability to convert malonyl-CoA into specific triketide products with notable kinetic preferences.
- Findings highlight the structural and stereochemical details of the intermediates during polyketide synthesis, revealing the distinct roles of active site residues in the dehydratase and ketoreductase
