Myeloablative radiochemotherapy followed by autologous peripheral blood stem cell transplantation as first-line therapy in peripheral T-cell lymphomas: first results of a prospective multicenter study.

Peripheral T-cell lymphomas (PTCL) show a poor outcome following conventional chemotherapy. However, the role of high-dose therapy is still unclear. We initiated a prospective multicenter phase II study to evaluate the feasibility and the efficacy of myeloablative radiochemotherapy followed by autologous peripheral blood stem cell transplantation (APBSCT) as first-line treatment in PTCL.

Gene transfer in purified human hematopoietic peripheral-blood stem cells by means of electroporation without prestimulation.

Gene transfer in hematopoietic stem cells (HSCs) is an important tool, exploring regulation of the hematopoietic system and understanding the development and expansion of malignant cell clones. It is also a mandatory step for gene therapy of hematopoietic disorders. Although retroviral transduction of HSCs is effective, prestimulation of cells is generally required, also inducing differentiation of HSCs.