Comparison of the response of cultured osteoblasts and osteoblasts outgrown from rat calvarial bone chips to nonfouling KRSR and FHRRIKA-peptide modified rough titanium surfaces.

Mimicking proteins found in the extracellular matrix (ECM) using specific peptide sequences is a well-known strategy for the design of biomimetic surfaces, but has not yet been widely exploited in the field of biomedical implants. This study investigated osteoblast and, as a control, fibroblast proliferation to novel consensus heparin-binding peptides sequences KRSR and FHRIKKA that were immobilized onto rough (particle-blasted and chemically etched) commercially pure titanium surfaces using a poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) molecular assembly system. This platform enabled a detailed study of specific cell-peptide interactions even in the presence of serum in the culture medium; thanks to the excellent nonfouling properties of the PLL-g-PEG surface.

Fabrication of TiO2-coated epoxy replicas with identical dual-type surface topographies used in cell culture assays.

The goal of this study was to reproducibly generate samples with complex surface topographies and chemistries identical to a "master surface" and to test their response in cell culture using rat calvarial cells. Negative replicas of dual-type topography were fabricated using dental impression material with half of the surface exhibiting smooth and rough topography, respectively. Positive epoxy resin replicas were cast from the same negative replica eight times consecutively and coated with a 60-nm thin film of titanium dioxide using a vapor deposition technique.

Systematic study of osteoblast response to nanotopography by means of nanoparticle-density gradients.

Features over a wide range of length scales affect the biological response to a surface. While the influence of micro-features has been extensively studied, the effect of nano-features has only rarely been systematically investigated. We have developed a simple method to produce nano-featured gradients by kinetically controlled adsorption of negatively charged silica nanoparticles onto positively charged, poly(ethylene imine) (PEI)-coated silicon wafers.

Functionalizable Nanomorphology Gradients via Colloidal Self-Assembly.

We present a novel approach for the fabrication of tailored nanomorphology gradients on metal oxide surfaces. We first show the direct formation of a nanocolloidal density gradient by a dip-coating process. The obtained silica nanoparticle gradients are then subjected to a heat treatment.

Systematic study of osteoblast and fibroblast response to roughness by means of surface-morphology gradients.

The surface roughness of a medical implant is of great importance since the surface is in direct contact with the host tissue (e.g. bone, fibrous tissue).

Material properties of and tissue reaction to the Slocum TPLO plate.

Objective: To determine the material properties of Slocum TPLO plates and assess the soft tissue reaction adjacent to these plates in dogs that had undergone tibial plateau leveling osteotomy (TPLO).

Sample Population: 3 new TPLO plates, 8 retrieved TPLO plates, and 1 new Synthes dynamic compression plate.

Procedures: Metallurgic analyses were performed.