Systematic probing of protein adsorption on protein-based nanoparticles in dependence of the particle surface charge.

Understanding protein adsorption on the surface of nanoparticles (NPs) is crucial for determining their behavior in biological environments. Early research in this field faced challenges in producing high-quality NPs. Advancements in NP fabrication now allow for precise modifications of specific parameters, such as zeta potential.

Overcoming Non-Specific Interactions for Efficient Encapsulation of Doxorubicin in Ferritin Nanocages for Targeted Drug Delivery.

Due to its beneficial pharmacological properties, ferritin (Ftn) is considered as an interesting drug delivery vehicle to alleviate the cardiotoxicity of doxorubicin (DOX) in chemotherapy. However, the encapsulation of DOX in Ftn suffers from heavy precipitation and low protein recovery yield which limits its full potential. Here, a new DOX encapsulation strategy by cysteine-maleimide conjugation is proposed.