Posterior stabilization in total knee arthroplasty with use of an ultracongruent polyethylene insert.

Fifty-three primary and 47 revision posterior cruciate ligament (PCL)-substituting total knee arthroplasties (TKAs) using a highly conforming (ultracongruent) polyethylene insert were retrospectively reviewed over a 48- to 106-month (mean, 60+/-11 months) follow-up period. These 100 knees were age and sex matched with another 100 TKAs performed using a PCL-sparing design. The ultracongruent design has an anterior buildup of 12.

Multiple transcripts of the murine immunoglobulin epsilon membrane locus are generated by alternative splicing and differential usage of two polyadenylation sites.

The human C epsilon gene produces a number of alternatively spliced heavy chain transcripts of which some encode functional IgE isoforms. We now show that differentially processed epsilon mRNA variants also exist in the mouse and are generated by differential polyadenylation and alternative splicing of primary epsilon chain transcripts. The two poly(A) sites of the mouse membrane transcripts were identified in the present study by RACE-PCR analysis.

Patellar component medialization in total knee arthroplasty.

Intraoperative correction of patellar maltracking has traditionally involved the use of a lateral retinacular release. Problems, however, related to lateral retinacular release include increased postoperative pain and wound healing complications, compromised patellar blood flow, and longer rehabilitation. The purpose of this study was to assess the effect of patellar medialization in total knee arthroplasty.

6- to 10-year experience using countersunk metal-backed patellas.

Three hundred two consecutive cementless total knee arthroplasties (Natural Knee, Intermedics Orthopedics, Inc., Austin, TX) were performed using a metal-backed, porous-coated patellar component. Fifty-nine patients died and 31 were lost to follow-up evaluation, resulting in 212 knees available for evaluation at 6 to 10 years.

Expression of CD44 splice variants in metastatic and non-metastatic mouse tumour cell lines.

Different splice variants of the CD44 cell-surface molecule have been linked to metastasis formation in several animal and human cancers. We have used metastatic CSML-100 and non-metastatic CSML-0 mouse adenocarcinoma cell lines to determine whether variant CD44 molecules could be implicated in the different behaviour of these cells. Two CD44 splice variants containing exons v7-v10 and v8-v10 were detected in the non-metastatic CSML-0.

Characterization of the human immunoglobulin epsilon mRNAs and their polyadenylation sites.

Several IgE heavy (H) chain transcripts are produced by alternative splicing between constant region (CH3 and CH4) and membrane (M1 and M2) exons and by differential cleavage-polyadenylation at poly(A) sites downstream of the CH4 and M2 exons. We have now characterized the poly(A) signal of the epsilon transcripts that contain membrane exon sequences (epsilon CH4-M1'-M2, epsilon CH4-M1-M2, epsilon CH4-M2' and epsilon CH4-M2") and have determined the complete sequence of the M2 exon and 1.4 kb of downstream genomic DNA.

Treatment of infected total knee arthroplasty using an articulating spacer.

Twenty-six patients with late infected total knee arthroplasties were treated by debridement and removal of components and all cement, preserving collateral ligaments. At time of debridement, an articulating spacer was fashioned to allow partial weightbearing and knee range of motion (ROM) during rehabilitation. This spacer was implanted using antibiotic-impregnated bone cement.

Staying on top--9 rules for surviving and excelling on the streets.

When EMS providers at both the BLS and ALS levels leave their training programs, they are armed with newfound knowledge, but they usually lack the appropriate life experiences to excel and survive on the streets. As these new providers enter the real world of EMS, they face the challenge of making life-and-death decisions in uncontrolled environments. This includes making the appropriate decisions while functioning effectively--even in potentially dangerous settings.

[The Bos taurus casein genes. Isolation and characterization of the kappa-casein gene].

A region spanning 25 kb of genomic DNA containing the kappa-casein gene, has been isolated from two genomic libraries in EMBL3 and EMBL4 phage vectors. Five phage clones containing kappa-casein gene have been found. Gene organisation has been determined using restriction mapping and a partial sequencing the 5' and 3' flanking regions.

Isolation and characterization of the bovine kappa-casein gene.

1. The bovine kappa-casein gene has been isolated as a series of overlapping lambda clones and shown to consist of five exons distributed over a total length of approximately 13 kb. Most of the mature protein-coding sequence is contained in a single large exon.

Isolation and characterization of the Bos taurus beta-casein gene.

The expression of casein genes in the mammary cells is regulated by peptide and steroid hormones. To investigate the controlling mechanisms we have isolated and characterized the bovine beta-casein gene. The gene has the size of 8.

[The casein genes of Bos taurus. II. Isolation and characteristics of the beta-casein gene].

The expression of the casein genes in mammary gland cells is regulated by peptide and steroid hormones. To study underlying regulatory mechanisms, the bovine beta-casein gene was isolated and characterized from lambda bacteriophage bovine DNA library. The beta-casein gene is 8.

[Antiproliferative action of swine leukocyte interferon and of an inhibitor of interferon action].

The results of the study of the antiproliferative effect of swine leukocyte interferon and of an inhibitor of interferon effect in experimental mice with transplanted Krebs-2 ascitic carcinoma cells are presented. The interferon inhibitor exerted antiproliferative effect similar to that of native swine interferon. Combined use of swine interferon and interferon inhibitor did not lead to summation of the antiproliferative effect of these preparations.

[Production of inhibitors of interferon action by cell lines sensitive and insensitive to the antiviral action of interferon].

The capacity of a number of human and murine cell cultures to produce, upon virus induction of interferon synthesis, low molecular admixtures inhibiting interferon antiviral activity was studied. The cells resistant to interferon were found to be 8-16 times as active producers of these admixtures as sensitive cells. A possible role of the production of these admixtures in functional, i.

[Inactivation of the inductor virus (Newcastle disease virus) during the isolation of porcine leukocyte interferon].

The use of the routine method for inactivation of the inductor virus by acidification of interferon to pH 2.0 resulted in a significant decrease in the antiviral activity of pig leukocytic interferon, since the preparation was highly sensitive to changes in the pH values. The use of 0.

[Concentration of heterogeneous leukocyte interferon].

A comparative study of different methods for concentration of porcine leukocyte interferon active in human cells was carried out. Ultrafiltration in an apparatus TCF-10 was found to be the most effective method of concentration by which preparations of porcine interferon showing antiviral activity up to 1 X 10(6) IU/ml were obtained. The total antiviral activity of concentrated interferon was twice as high as that of the native preparation.