[The use of intraaortic balloon counterpulsation in patients with acute myocardial infarction].

Intraaortic balloon counterpulsation was implemented in 11 patients with acute myocardial infarction and 1 patient with unstable angina. All patients had severe multivessel coronary artery disease. In 9 patients counterpulsation was used in conjunction with percutaneous transluminal coronary angioplasty, in 3 - with thrombolytic therapy.

[The antioxidant prevention of disorders in calcium ion metabolism under the action of glutamate on the synaptosomes of the rat cerebral cortex].

An increase of intracellular calcium ion concentration and of the 45Ca2+ entry, a decrease in Na+,K(+)-ATPase activity, and activation of Na+/Ca2+ exchange were shown to be initiated by glutamate in the rat brain cortex synaptosomes. These effects could be prevented with antagonists and blocking agents of the NMDA receptors. Pre-incubation of the synaptosomes with alpha-tocopherol, superoxide dismutase, and ganglioside GM1 was shown to normalise [45Ca2+], the rate of 45Ca2+ entry, and the activity of Na+,K(+)-ATPase in the synaptosomes.

[The effect of bradykinin, vasopressin and ACTH (4-10) on the morphine withdrawal syndrome in morphine-dependent rats of 2 inbred strains].

The effect of intranasal administration of bradykinin, ACTH(4-10) fragment, and vasopressin on the withdrawal syndrome was studied in morphine-dependent rats of two inbred strains, WAG/G and Fischer-344. Morphine dependence was stronger and the withdrawal syndrome had a more severe course in WAG/G rats. Intranasal administration of bradykinin and the ACTH(4-10) fragment in the dose used had no effect on the expression of the withdrawal syndrome in rats of both strains.

[The repair of the membrane lipid bilayer in oxidative stress: phosphatidylethanolamine reacylation in synaptosome, photoreceptor and erythrocyte membranes].

The participation of unsaturated (linoleic and arachidonic) and saturated (palmitic) fatty acids in reacylation of phosphatidylethanolamine (PE) in synaptosomes, photoreceptor membranes and erythrocytes at oxidative stress was studied. Induction of lipid peroxidation (LPO) was found to result in a significant decrease in the content of PE polyenoic fatty acids due to their oxidative destruction. It might be related to both an activation of phospholipase A2 and a decrease in PE reacylation rate.

[The genetic characteristics of the analgesic action of substance P, bradykinin and thyroliberin in the rats of 2 inbred strains].

A participation of nonopiate peptide brain systems in the mechanism of individual pain sensation was studied. The experiments were carried out on rats of two inbred strains, Fischer-344 and WAG/G, which exhibit different threshold of thermal pain sensation. The action of nonopiate oligopeptides (substance P, bradykinin, and thiroliberin) on the latent period of tail immersion from hot water was studied.

[The genetic characteristics of the brain alpha 2-adrenergic and S2-serotoninergic systems in the realization of the tail-jerk pain reflex and its morphine suppression in rats].

Experiments were conducted on two inbred rat strains--WAG/G and Fischer-344. The WAG/G rats were found to increase the density of brain cortical mu-opiate and alpha 2-adrenergic receptors, short latency of tail immersion from hot water, decreased analgesic action of 5 mg/kg of intraperitoneal morphine and pronounced analgesic action of 10 micrograms of intracerebral ventricular clonidine The Fischer-344 rats increased S2-receptor affinity, protracted pain latency, increased the analgesic action of morphine (5 mg/kg i.p.

[The pharmacological properties of karazedin].

The original agent 1,2,3,4,5,5a,6,10b-octahydroazepino-[4,5-b]indole (carazedine), an analog of the highly active antipsychotic drug carbidine, was synthesized. This agent was found to have various psychotropic activities and to be superior to carbidine, as shown by a number of tests, and combine properties typical of neuroleptics and tranquilizers. The toxicity of carazedine and carbidine was equal.

[Ganglioside participation in the regulation of free-radical reactions in brain membranes].

The effect of monosialoganglioside GM1 on induced free radical reactions in the synaptosomal and myelin membranes induced by Fe(2+)-H2O2 system was studied. The formation of free radicals was determined by measuring luminol-dependent chemoluminescence. It was found that preincubation of the membranes with GM1 (10(-11)-10(-6) M) and 12 or 12-palmitate, 13-acetate phorbol ester (10(-7)-10(-6) M) or alpha-tocopherol (10(-6) M) results in the decrease of chemiluminescent response.

[Ganglioside protection of the erythrocyte membranes in myocardial ischemia].

Myocardial ischemia was shown to lead to modification of structural and functional organization of rat erythrocyte membranes. Thus, it was found that the activity of Na+, K+-ATP-ase markedly decreased, while accumulation of LPO products and of lysophosphatidylcholine (lyso--PC) took place in erythrocyte membranes of rats subjected to myocardial ischemia. Using nonpenetrating modifier trinitrobenzosulfonic acid, an increase in the content of modified phosphatidylethanolamine in erythrocyte membranes of ischemic rats was revealed as compared to the membranes of control animals.

[An assessment of the individual sensitivity of Wistar rats to the development of morphine dependence].

A search was made for behavioral and physiological indicators which could serve predictors of the individual sensitivity of Wistar rats to the development of physical morphine dependence. Animals with high sensitivity to the development of dependence initially demonstrated intensive ambulation and low motor rearing in the "open field" and had low nociception. Stable animals exhibited high ambulation and motor rearing with insignificant grooming activity as compared to other rats.

[Neurotropin suppression of the manifestations of the naloxone-provoked withdrawal syndrome in morphine-dependent rats].

Neurotropin was developed by Nippon Zoki Pharmaceutical Co. Ltd. It is produced by inoculating vaccine virus into rabbits and the effective substances are extracted from neuro-immuno inflamed cutaneous tissue.

[Participation of gangliosides in protection of beta adrenergic receptors from damaging effect induced by lipid peroxidation in synaptosome membranes].

The induction of lipid peroxidation (LPO) in rat brain synaptosomes was shown to result in considerable decrease of the level of specific [3H]-dihydroalprenolol binding, decrease of Bmax and increase of KD. It was revealed that the preincubation of rat brain synaptosomes with monosialoganglioside GM1 (10(-8) M) or alpha-tocopherol (10(-6) M) led to a decrease in MDA accumulation after LPO induction by Fe(2+)-ascorbate system. AT the same time GM1 prevents damage of beta-adrenoreceptors, caused by LPO induction, having no effect on the functional state of beta-adrenoreceptors in control preparations.

[Ganglioside-dependent factor inhibiting lipid peroxidation in synaptosomal membranes].

The inhibitory effect of exogenous monosialoganglioside GM1 on lipid peroxidation was studied in synaptosomal membranes from rat brain. When this effect was studied over a wide GM1 concentration range, the biphasic kinetics was observed, the highest per cent of inhibition (70%) was found at GM1 concentration of 10(-9)- 10(-8) M. In liposomes made from lipids isolated from rat synaptosomal membranes the inhibition of lipid peroxidation by exogenous GM1 was much less pronounced (25% at maximum) it reached the saturation at ganglioside concentration of 10(-8)-10(-6) M.

[Method for evaluating the reinforcing properties of morphine in a model of addictive behavior].

An animal model of addiction is described. The albino rats learned to come into the right arm of the Y-type maze where they received intraperitoneal injections of morphine in a dose of 5 mg/kg or distilled water (control group). It was found that morphine-dependent rats much more frequently visited the right arm.