Rosiglitazone quantification in rat plasma using high performance liquid chromatography with mass spectrometric detection and its application to pre-clinical pharmacokinetic studies.

Unlabelled: A rapid and sensitive high performance liquid chromatography-mass spectrometry method has been developed for quantitative determination of Rosiglitazone, a thiazolidinedione drug used for the treatment of type II diabetes mellitus in rat plasma. The method was also validated as per International Conference on Harmonization (ICH) and Food and Drug Administration (FDA) guidelines. The analyte was extracted from rat plasma by the simple precipitation of plasma proteins technique using acetonitrile as a precipitating agent.

Separation and quantification of eight antidiabetic drugs on a high-performance liquid chromatography: its application to human plasma assay.

An analytical method based on isocratic reverse phase high-performance liquid chromatography was developed and validated for the separation and quantification of eight antidiabetic drugs: rosiglitazone, pioglitazone, glipizide, gliclazide, repaglinide, nateglinide, glibenclamide, and glimepiride for their application in human plasma assay. Metformin is used as internal standard. Analysis was done on Onyx monolithic C(18) column (100 × 4.