Publications by authors named "Tirthankar Biswas"
Article Synopsis
- - Genetically-encoded single-cell barcodes can help with tasks like tracing cell lineages and conducting genetic screens, but current methods have limitations in speed and effectiveness.
- - This study proposes a new method using combinations of fluorescent proteins to create a high-diversity barcode library that allows for non-destructive, quick, and affordable identification of cells.
- - The researchers successfully tested this method, creating a library of about 150 unique barcodes using 18 fluorescent proteins, and demonstrated its effectiveness for classifying cells and enabling genetic screenings and lineage tracing.
The rice, like other plants, undergoes photoprotection mode by increasing nonphotochemical quenching (NPQ) in high light intensity (> 1200 µmol ms PPFD), which attenuates photosystem II yield (φPSII) drastically. The plant remains in photoprotection mode even after light intensity becomes not stressful for an extended period. While there are significant differences in the time it takes for photoprotection to recover among different genotypes, its use is limited in plant breeding because measuring the chlorophyll fluorescence parameters in progressive actinic light after dark adaptation takes more than forty-five minutes per genotype.
View Article and Find Full Text PDFRice is one of the major food crops grown globally. However, during the wet season, rice suffers significant yield loss due to reduced light intensity caused by overcast clouds when the light intensity is only around 450-500 µmol/m/s, compared to 1400-1800 µmol/m/s in summer. This reduction in light intensity leads to a decrease in seed yield, mainly by limiting tiller or panicle numbers.
View Article and Find Full Text PDFThe physiological mechanisms of shade tolerance and trait plasticity variations under shade remain poorly understood in rice (Oryza sativa L.). Twenty-five genotypes of rice were evaluated under open and shade conditions.
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- Identified a new SPIKE-allele from the rice variety Bhutmuri that enhances the number of spikelets without reducing panicle numbers, critical for improving rice yields.
- Developed a specific PCR-based marker for easy genotyping, targeting a single nucleotide polymorphism (SNP) linked to the SPIKE allele.
- Bhutmuri variety showed significantly higher SPIKE transcript levels and enhanced photosynthesis rates compared to the IR64 variety, indicating its potential for use in marker-assisted breeding to boost rice productivity.
The development of low arsenic-accumulating varieties for the contaminated areas is one of the best options for reducing the dietary exposure of arsenic to human population through rice. In this study, grain-arsenic content in one hundred genotypes revealed a large variation ranging from 0.05 mg/kg to 0.
View Article and Find Full Text PDFPhosphate availability is a major factor limiting tillering, grain filling vis-á-vis productivity of rice. Rice is often cultivated in soil like red and lateritic or acid, with low soluble phosphate content. To identify the best genotype suitable for these types of soils, P acquisition efficiency was estimated from 108 genotypes.
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