Publications by authors named "Tiphaine Legrand"
Article Synopsis
- Heart transplantation is crucial for patients with advanced heart failure, but there's a shortage of donor organs, leading to the increased use of extended criteria donors (ECD).
- Many ECDs at risk for coronary artery disease do not undergo standard coronary angiography, resulting in some hearts being declined or procured without proper screening.
- The described protocol demonstrates a new method for performing coronary angiography during normothermic ex-situ heart perfusion (NESP), showing it to be reproducible without affecting heart viability and potentially improving ECD procurement rates.
Determination of hydroxyurea in human plasma by HPLC-UV using derivatization with xanthydrol.
J Chromatogr B Analyt Technol Biomed Life Sci
October 2017
A simple and rapid high performance liquid chromatography (HPLC) method using ultraviolet (UV) detection was developed to determine hydroxyurea (HU) concentration in plasma sample after derivatization with xanthydrol. Two hundred microliters samples were spiked with methylurea (MeU) as internal standard and proteins were precipitated by adding methanol. Derivatization of HU and MeU was immediately performed by adding 0.
View Article and Find Full Text PDFA simple and rapid ultra-high-performance liquid chromatography (UHPLC) method using UV detection was developed for the simultaneous determination of eight β-lactam antibiotics in human plasma, including four penicillins, amoxicillin (AMX), cloxacillin (CLX), oxacillin (OXA), and piperacillin (PIP), and four cephalosporins, cefazolin (CFZ), cefepime (FEP), cefotaxime (CTX), and ceftazidime (CAZ). One hundred-microliter samples were spiked with thiopental as an internal standard, and proteins were precipitated by acetonitrile containing 0.1% formic acid.
View Article and Find Full Text PDFIn clinical practice, rifampicin exposure is estimated from its concentration in venous blood samples. In this study, we hypothesized that differences in rifampicin concentration may exist between arterial and venous plasma. An HPLC-UV method for determining rifampicin concentration in plasma using rifapentine as an internal standard was validated.
View Article and Find Full Text PDFBackground: Studies suggest that particular antimicrobial and anti-inflammatory properties of azithromycin (AZM) can be related to its extensive accumulation in white blood cells (WBCs). However, available methods for determination of AZM in WBCs require large blood volumes unsuited to a pediatric context. Therefore, an LC-MS/MS method was developed for determination of AZM in WBCs.
View Article and Find Full Text PDFSimultaneous determination of three carbapenem antibiotics in plasma by HPLC with ultraviolet detection.
J Chromatogr B Analyt Technol Biomed Life Sci
November 2008
A simple, precise and accurate high-performance liquid chromatography (HPLC) method using ultraviolet (UV) detection has been developed for simultaneous determination of carbapenem antibiotics: imipenem, meropenem and ertapenem in human plasma. Samples were spiked with ceftazidime as internal standard and proteins were precipitated by acetonitrile. Separation was achieved on a C8 column with a mobile phase composed of phosphate buffer 0.
View Article and Find Full Text PDFObjective: The objective of this study was to analyze in a pediatric hospital the use of expensive drugs as part of the new activity-based system (T2A) of funding for French public hospitals. We identified and analyzed the therapeutic use of these drugs in indications not included in the expert recommendations issued to accompany this change, with the goal of proposing specific pediatric recommendations.
Method: Analysis of prescriptions from May through September 2005 showed that 259 patients received expensive drugs subject to special reimbursement.