[Forensic Investigation of Goldeneye™ DNA ID 22NC Kit].

Objective: To investigate the genetic data of 21 autosomal STR included in Goldeneye™ DNA ID 22NC Kit in Chinese Han nationality and to evaluate the forensic application.

Methods: By detected 500 unrelated healthy individuals in Chinese Han nationality of East China with Goldeneye™ DNA ID 22NC Kit, allele frequencies, population genetics parameters and linkage disequilibrium information of the 21 autosomal STR were statistically analyzed.

Results: In the 21 autosomal STR, no deviations from Hardy-Weinberg equilibrium were detected and all loci were independent form each other.

A case of false mother included with 46 autosomal STR markers.

Background: For solving a maternity case, 19 autosomal short tandem repeats (STRs) were amplified using the AmpFℓSTR(®) Sinofiler(TM) kit and PowerPlex(®) 16 System. Additional 27 autosomal STR loci were analyzed using two domestic kits AGCU 21+1 and STRtyper-10G. The combined maternity index (CMI) was calculated to be 3.

[Forensic validation of goldeneye? DNA ID 26Y system].

Objective: To perform the validation and analysis of forensic parameters of Goldeneye DNA ID 26Y system.

Methods: Based on the validation rules of Scientific Working Group on DNA Analysis Methods (SWGDAM), the kit was assessed from several parts, as test of PCR system, reproducibility, accuracy, and sensitivity, etc. And Y-STR loci of 517 unrelated healthy individuals from Eastern China were genotypes by this kit.

[Universal algoritihms for paternity index in trios and its extended application].

Objective: To introduce an universal algorithm for kinship index between a baby and a random person with biologic mother reference.

Methods: Based on the formulas of paternity index in trios (PIT), common factors shared in these formulas were deduced following reconstructions of these formulas with the common factors. Universal algorithms for other common kinship indices, such as grandparental index (GI), half sibling index (HSI), avuncular index (AI) and first cousin index (CI1st), were investigated according to avuncular index rule and the coefficient of relationship (r).

[Establishment of universal algorithms for commonly used kinship indices between two individuals].

Objective: To establish universal algorithms for commonly used kinship indices between two individuals.

Methods: Based on the formulas of paternity index in duos(PID), full sibling index(FSI), half sibling index (HSI), avuncular index (AI), grandparental index (GI) and first cousin index (CI1st) deduced from ITO method, the common factors, 1 plus reciprocal of the frequency of the allele with identity by state between the two individuals, shared in these formulas were abstracted with induction method, following with reconstruction of these formulas with the common factor and the coefficient of relationship (r).

Results: A universal algorithm for PI(D), HSI, AI, GI and CI1st, was developed with the common factor and r value according to the heterozygosity of the two individuals.

[Application of multiple polymorphism genetic markers in determination of half sibling sharing a same mother].

Objective: Determination strategies for half sibling sharing a same mother were investigated through the detection of autosomal and X-chromosomal STR (X-STR) loci and polymorphisms on hypervariable (HV) region of mitochondrial DNA (mtDNA).

Methods: Genomic DNA were extracted from blood stain samples of the 3 full siblings and one dubious half sibling sharing the same mother with them. Fifteen autosomal STR loci were genotyped by Sinofiler kit, and 19 X-STR loci were genotyped by Mentype Argus X-8 kit and 16 plex in-house system.

[Application of the number of allele shared among autosomal STR loci in full sibling identification].

Objective: To establish and evaluate the method of full sibling identification based on the number of allele shared among autosomal STR Loci.

Methods: Two hundred and eighty full sibling pairs and 2,003 unrelated individual pairs were genotyped in 15 STR loci with Identifiler Kit, and the number of allele shared among the 15 STR loci (S15) and full sibling index (FSI) were calculated. Fisher discriminant functions were established with SAS 8.

[The evaluation of Identifiler system in paternity testing].

Objective: To evaluate the power of Identifiler System for paternity testing.

Methods: A total of 3 277 paternity testing cases were studied using Identifiler System. The exclusion power and mutation rates of the Identifiler System were analysed in the paternity testing.

Genetic polymorphism of 17 STR loci for forensic use in Chinese population from Shanghai in East China.

Allele frequencies for 17 STR loci found in Identifier kit and PowerPlex16 Monoplex System were determined in a sample of 1000 unrelated individuals living in Shanghai in East China. The values of observed heterozygosity (Ho), power of discrimination (PD), probability of paternity exclusion (PE) and polymorphism information content (PIC) were calculated. All loci were in accordance with Hardy-Weinberg equilibrium (p<0.

[Analysis of appropriate amount of template DNA for sinofiler kit by real time quantitative PCR technique].

Objective: To explore the appropriate amount of template DNA for Sinofiler Kit.

Methods: The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.

Results: It was shown that 1.

[Application of D6S1043 and D12S391 loci in forensic paternity testing].

Objective: The aim was to investigate the polymorphisms of D6S1043 and D12S391 loci among Han population and evaluate their values in paternity testing. MERTHODS: By using fluorescence dye-labeled primers and capillary electrophoresis, the allele frequencies of the two STR loci among 192 unrelated individuals were investigated.

Results: Twelve alleles were observed in both D6S1043 and D12S391 loci.

SNP genotyping by multiplex amplification and microarrays assay for forensic application.

Objective: Research on the application feasibility of SNP genotyping for forensic identification by microarrays.

Methods: Oligonucleotide microarrays which could detect 34 different SNPs were used. After hybridization and washing, the arrays were scanned and fluorescence intensities analyzed using Microarray software.

[Developing of a new multicolor-fluorescent labeled STR amplification kit].

Objective: To develop a PCR-based STR system for genotyping of 18 loci (Amelogenin, D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D16S539, TH01, TPOX, CSF1PO, D7S820, D2S1338, D19S433, D12S391 and D19S253).

Methods: By using primers labeled with four color fluorescent (FAM, HEX, TAMRA and ROX), two multiplex amplification reaction systems were developed to genotype Amelogenin and 17 STR loci.

Results: Amelogenin and these 17 STR loci were genotyped successfully in different kinds of biological samples by the kit.

[ABO genotyping by duplex amplification and oligonucleotide arrays assay].

Objective: ABO genotyping for forensic identification by oligonucleotide chip.

Methods: Oligonucleotide microarrays which could detect 3 different SNPs in exon 6 and exon 7 for ABO genotyping were used. Population studies on ABO was carried out in a sample of 115 unrelated Chinese Han individuals.