Functional characterization of complement factor H in host defense against bacterial pathogen in Nile tilapia (Oreochromis niloticus).

Complement factor H (CFH), a multifunctional soluble complement regulatory protein, can bind to a variety of pathogens and play a crucial role in host innate immune defense. To explore the functional characteristics of CFH (OnCFH) in Nile tilapia (Oreochromis niloticus), we cloned and characterized the open reading frame (ORF) of OnCFH in this study. The full-length of OnCFH ORF is 1359 bp, encoding 452 aa for a 48.

Europium(III) modified silicone nanoparticles for ultrasensitive visual determination of tetracyclines by employing a fluorescence color switch.

Silicon nanoparticles (SiNPs) modified with Eu(III) were synthesized and are shown to be a viable ratiometric fluorescent probe for tetracycline antibiotics. SiNPs/Eu under 405 nm excitation display two emissions, viz. a strong cyan colored fluorescence peaking at 497 nm and a weak pink fluorescence peaking at 622 nm.

Modification-free carbon dots as turn-on fluorescence probe for detection of organophosphorus pesticides.

It is important to detect pesticides residues due to the concern over food safety. In this work, the burning ash of waste paper was used as carbon source to synthesize carbon dots (C-dots). The fluorescence of obtained C-dots could been turn off by Fe which was from Fe oxidized by HO, organophosphorus pesticides could effectively inhibit the production of HO by destroying the acetylcholinesterase activity, so the fluorescence of C-dots hold turning on in the presence of organophosphorus pesticides.