Convergent evolution of berberine biosynthesis.

Berberine is an effective antimicrobial and antidiabetic alkaloid, primarily extracted from divergent botanical lineages, specifically (Ranunculales, early-diverging eudicot) and (Sapindales, core eudicot). In comparison with its known pathway in species, its biosynthesis in species remains elusive. Using chromosome-level genome assembly, coexpression matrix, and biochemical assays, we identified six key steps in berberine biosynthesis from , including methylation, hydroxylation, and berberine bridge formation.

Cloning, Expression Characteristics of Gene from and Functional Identification in Triterpenoid Synthesis.

is a medicinal plant whose main component is platycodins, which have a variety of pharmacological effects and nutritional values. The farnesyl pyrophosphate synthase (FPS) is a key enzyme in the isoprenoid biosynthesis pathway, which catalyzes the synthesis of farnesyl diphosphate (FPP). In this study, we cloned the gene from () with an ORF of 1260 bp, encoding 419 amino acids with a deduced molecular weight and theoretical pI of 46,200.

LC-MS and MALDI-MSI-based metabolomic approaches provide insights into the spatial-temporal metabolite profiles of Tartary buckwheat achene development.

Tartary buckwheat, celebrated as the "king of grains" for its flavonoid and phenolic acid richness, has health-promoting properties. Despite significant morphological and metabolic variations in mature achenes, research on their developmental process is limited. Utilizing Liquid chromatography-mass spectrometry and atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry imaging, we conducted spatial-temporal metabolomics on two cultivars during achene development.

A transcriptional complex of FtMYB102 and FtbHLH4 coordinately regulates the accumulation of rutin in Fagopyrum tataricum.

Tartary buckwheat is rich in flavonoids, which not only play an important role in the plant-environment interaction, but are also beneficial to human health. Rutin is a therapeutic flavonol which is massively accumulated in Tartary buckwheat. It has been demonstrated that transcription factors control rutin biosynthesis.

Profiling of polyphenols for in-depth understanding of Tartary buckwheat sprouts: Correlation between cultivars and active components, dynamic changes and the effects of ultraviolet B stress.

Tartary buckwheat sprouts have a high nutritional value and are gluten-free, and polyphenols are their main active constituents. However, information regarding the active constituents' difference of Tartary buckwheat sprouts grown from seeds with different morphology, at different developmental stages and environments is limited. Here, we developed a LC-MS-based targeted metabolomics approach to analyze polyphenols (46 flavonoids and 6 anthraquinones) in 40 Tartary buckwheat sprouts varieties.

Promoting direct interspecies electron transfer and acetoclastic methanogenesis for enhancing anaerobic digestion of butanol octanol wastewater by coupling granular activated carbon and exogenous hydrogen.

Butanol octanol wastewater (BOW) generated from syngas conversion of coal contained abundant toxic organic pollutants. Anaerobic digestion is a promising technology for BOW, but abundant toxic substances would inhibit the activity of anaerobic microorganisms. Granular activated carbon (GAC) and exogenous hydrogen (EH) were employed to enhance anaerobic digestion of BOW.

Coupling granular activated carbon and exogenous hydrogen to enhance anaerobic digestion of phenol via predominant syntrophic acetate oxidation and hydrogenotrophic methanogenesis pathway.

Anaerobic digestion is a promising biological method for treating phenol-containing wastewater. However, the low methane yield of phenol due to the biological toxicity limits its potential application. This study presents a novel method to enhance the conversion rate of phenol to methane by coupling of granular activated carbon and exogenous hydrogen (GAC/H).

A POCS super resolution restoration algorithm based on BM3D.

The inherent shortcoming of POCS (Projection Onto Convex Sets) is its sensitiveness to noise. The restoration quality of POCS based super resolution will severely decline when the noise is larger. In practical applications, the low resolution images generally include some kinds of noise, such as camera internal noise, transmission system noise and coherent noise.