Light-Sheet Skew Ray-Based Microbubble Chemical Sensor for Pb Measurements.

A multimode fiber-based sensor is proposed and demonstrated for the detection of lead traces in contaminated water. The sensing mechanism involves using a light sheet to excite a specific group of skew rays that optimizes light absorption. The sensing region features an inline microbubble structure that funnels the skew rays into a tight ring, thereby intensifying the evanescent field.

Prevalence, Genotype Diversity, and Distinct Pathogenicity of 205 Gammacoronavirus Infectious Bronchitis Virus Isolates in China during 2019-2023.

Gammacoronavirus infectious bronchitis virus (IBV) causes a highly contagious disease in chickens and seriously endangers the poultry industry. The emergence and co-circulation of diverse IBV serotypes and genotypes with distinct pathogenicity worldwide pose a serious challenge to the development of effective intervention measures. In this study, we report the epidemic trends of IBV in China from 2019 to 2023 and a comparative analysis on the antigenic characteristics and pathogenicity of isolates among major prevalent lineages.

In vitro immunoregulatory role of recombinant Ancylostoma ceylanicum calreticulin.

Ancylostoma ceylanicum is a zoonotic soil-derived nematode that parasitizes the intestines of humans and animals (dogs and cats), leading to malnutrition and iron-deficiency anemia. Helminth parasites secrete calreticulin (CRT), which regulates or blocks the host's immune response. However, no data on A.

Phylogenetic relationship of Prohemistomum vivax to other trematodes based on the internal transcribed spacer region and mitochondrial genes.

Prohemistomum vivax is a zoonotic small cyathocotylid trematode that inhabits the intestines of fish-eating birds and mammals. Here, we amplified the internal transcribed spacer (ITS) sequence and six mitochondrial protein-coding genes (PCGs) from P. vivax.

Two-year imaging outcomes from a phase 3 randomized trial of secukinumab in patients with non-radiographic axial spondyloarthritis.

Background: Radiographic progression and course of inflammation over 2 years in patients with non-radiographic axial spondyloarthritis (nr-axSpA) from the phase 3, randomized, PREVENT study are reported here.

Methods: In the PREVENT study, adult patients fulfilling the Assessment of SpondyloArthritis International Society classification criteria for nr-axSpA with elevated CRP and/or MRI inflammation received secukinumab 150 mg or placebo. All patients received open-label secukinumab from week 52 onward.

Boosting the electrochemiluminescence of luminol by high-intensity focused ultrasound pretreatment combined with 1T/2H MoS catalysis to construct a sensitive sensing platform.

In the luminol-O ECL system, O as an endogenous coreactant has the advantages of non-toxicity and stability. Improving the efficiency to generate radicals of O is a challenge currently. In this work, a strategy combining physical method - ultrasound and nanomaterial with unique physicochemical properties was designed to enhance the ECL signal of luminol-O system.

Tumor-Derived Exosomal miR-29b Reduces Angiogenesis in Pancreatic Cancer by Silencing ROBO1 and SRGAP2.

Background: Exosomal miR-29b reportedly plays a role during cancer metastasis. However, its exact function and underlying mechanism during pancreatic cancer (PC) have not been investigated.

Methods: Exosomes from PC cells were prepared and identified.

Immune pathogenesis in pigeons during experimental infection.

is a small trematode belonging to the family Cyathocotylidae, infecting fish-eating birds and mammals, including humans. However, no data on molecular identification and immune pathogenesis are available, challenging effective diagnostic and therapeutic interventions. Here, we identified based on combined morphological and molecular data and examined histopathological lesions and the differential cytokines expression in experimentally infected pigeons.

Identification and Validation of Three Hub Genes Involved in Cell Proliferation and Prognosis of Castration-Resistant Prostate Cancer.

Background: The acquisition of castration resistance is lethal and inevitable in most prostate cancer patients under hormone therapy. However, effective biomarkers and therapeutic targets for castration-resistant prostate cancer remain to be defined.

Methods: Comprehensive bioinformatics tools were used to screen hub genes in castration-resistant prostate cancer and were verified in androgen-dependent prostate cancer and castration-resistant prostate cancer in TCGA and the SU2C/PCF Dream Team database, respectively.

Nano-hybrid luminophores of TiCT quantum dots-gold nanoparticles based on in situ generation for sensitive electrochemiluminescence biosensing.

Nanomaterial-derived quantum dots (QDs) are excellent electrochemiluminescence (ECL) luminophores and play an important role in optical sensing due to their excellent water solubility, good biocompatibility and tunable molecular size. In this work, a novel strategy was designed to form nano-hybrid TiC QDs-AuNPs in situ as a luminophore based on the unique reducibility of TiC QDs, which showed remarkable and stable ECL performance. Here, AuNPs were formed in situ without the addition of reducing agents and stabilizers, leading to threefold enhancement of the ECL signal of TiC QDs due to their excellent charge transfer capability.

Eukaryotic expression and immunogenicity of Ancylostoma ceylanicum calreticulin.

Ancylostoma ceylanicum is a zoonotic soil-derived nematode that parasitizes human and animal intestines, causing malnutrition and iron-deficiency anemia. Calreticulin is a multifunctional protein involved in all stages of parasitic infection. Studies have found that parasites can secret calreticulin to regulate the host's immune response.

Corrigendum: Anti-Tumoral Effect and Action Mechanism of Exosomes Derived From -Infected Dendritic Cells in Mice Colorectal Cancer.

[This corrects the article DOI: 10.3389/fonc.2022.

Anti-Tumoral Effect and Action Mechanism of Exosomes Derived From -Infected Dendritic Cells in Mice Colorectal Cancer.

is an obligate intracellular protozoan with anti-tumor activity against a variety of cancers. However, the therapeutic effect of on colorectal cancer is unclear, and using direct infection in immunotherapy involves safety concerns. This study investigated the anti-tumoral effect and mechanism of exosomes derived from dendritic cells (DCs) infected with (Me49-DC-Exo).

A Phase III Multicenter Randomized Clinical Trial of 60 Gy versus 50 Gy Radiation Dose in Concurrent Chemoradiotherapy for Inoperable Esophageal Squamous Cell Carcinoma.

Purpose: In this multicenter phase 3 trial, the efficacy and safety of 60 Gy and 50 Gy doses delivered with modern radiotherapy technology for definitive concurrent chemoradiotherapy (CCRT) in patients with inoperable esophageal squamous cell carcinoma (ESCC) were evaluated.

Patients And Methods: Patients with pathologically confirmed stage IIA‒IVA ESCC were randomized 1:1 to receive conventional fractionated 60 Gy or 50 Gy to the tumor and regional lymph nodes. Concurrent weekly chemotherapy (docetaxel 25 mg/m2; cisplatin 25 mg/m2) and two cycles of consolidation chemotherapy (docetaxel 70 mg/m2; cisplatin 25 mg/m2 days 1‒3) were administered.

Sensitive electrochemiluminescence biosensing of polynucleotide kinase using the versatility of two-dimensional TiCT MXene nanomaterials.

Electrochemiluminescence (ECL) is a powerful readout method for the development of (bio)sensors, whose performances depend on the electrode materials and the architecture of its surface. Herein, we demonstrate that the precise control of the sensing interface using the versatility of two-dimensional (2D) transition metal carbides (TiCT MXene) leads to the enhancement of the ECL signal. This electrode material, which exhibits remarkable structural and electrochemical properties was decorated by the in situ formation of gold nanoparticles (AuNPs) owing to the Ti reducibility.

Various potentially toxic element tolerances in different rice genotypes correlate with distinct physiological responses and alterations in DNA methylation.

Potentially toxic elements (PTEs) are harmful to plant growth and reduce crop productivity. In this work, we studied three rice genotypes (T-35, RZ-1, and RZ-2) to quantify the diverse PTE effects and tolerances by examining morphology, physiology, and DNA methylation patterns. Morphological results showed that T-35 exhibits the highest tolerance to all studied PTE stressors (Cu, Cd, Cr).

Alpha-terpineol grafted acetylated lentinan as an anti-bacterial adhesion agent.

Biomedical implants-associated bacterial infections have become a major threat to human health. Therefore, it is meaningful to develop new antibacterial strategies to solve this problem. In this study, we conjugated acetylated lentinan (AceLNT) with α-terpineol (AceLNT-g-α-ter), a highly effective natural antibacterial compound, to constitute a novel AceLNT-g-α-ter membrane (AceLNT-g-α-terM).

Anchoring luminol based on TiC-mediated in situ formation of Au NPs for construction of an efficient probe for miRNA electrogenerated chemiluminescence detection.

An efficient electrogenerated chemiluminescence (ECL) nanoprobe (luminol-Au NPs-TiC) was constructed based on TiCTx MXene (TiC)-mediated in situ formation of Au NPs and anchoring luminol to fabricate a sensitive ECL biosensor for miRNA-155 detection. Herein, TiC with rich Ti vacancy defects was used as reducing agent, and Au NPs were generated in situ and anchored on the TiC (Au NPs-TiC). Moreover, the Au NPs-TiC composites were used as a carrier and provided a large number of sites for the efficient linking of luminol through Au-N bonds to form stable luminol-Au NPs-TiC.

Thermo/glutathione-sensitive release kinetics of heterogeneous magnetic micro-organogel prepared by sono-catalysis.

To improve the loading and delivery for hydrophobic drugs and optimize the release efficiency in tumor microenvironment, a novel core-shell magnetic micro-organogel carrier was successfully prepared by a sono-catalysis process in the study. As-synthesized magnetic micro-organogel had an appropriate dispersibility in water owing to the hydrophilicity of protein shell and could be kept steadily with a well-defined spherical morphology owing to the three-dimensional gel structure of oil core, and it promised an accessible targeted drug delivery owing to its good magnetism-mediated motion ability. Moreover, the magnetic micro-organogel showed a high loading efficiency up to 94.

Expression and biological functions of Ancylostoma ceylanicum saposin-like protein.

Ancylostoma ceylanicum is a common zoonotic nematode that inhabits the small intestine of humans, dogs, and cats. Saposin-like proteins (SLPs) have hemolytic and antibacterial activities and could be used as diagnostic or vaccine candidates. To explore the biological functions of Ancylostoma ceylanicum SLP (Ace-SLP-1), cDNA-encoding Ace-SLP-1 mature peptide was cloned into prokaryotic expression vector pET-28a and transformed into Escherichia coli BL21 (DE3) to induce expression.

The mitochondrial genome sequence analysis of Ophidascaris baylisi from the Burmese python (Python molurus bivittatus).

Ophidascaris species are parasitic roundworms that inhabit the python gut, resulting in severe granulomatous lesions or even death. However, the classification and nomenclature of these roundworms are still controversial. Our study aims to identify a snake roundworm from the Burmese python (Python molurus bivittatus) and analyze the mitochondrial genome.

High concentrations of hypochlorous acid-based disinfectant in the environment reduced the load of SARS-CoV-2 in nucleic acid amplification testing.

During the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) pandemic, chlorine-containing disinfectants have been widely used in nucleic acid amplification testing laboratories. Whether the use of disinfectants affect the results of viral nucleic acid amplification is unknown. We examined the impact of different hypochlorous acid (HOCl) concentrations on the quantitative results of SARS-CoV-2 by real-time reverse-transcription polymerase chain reaction (RT-PCR).

A power-triggered preparation strategy of nano-structured inorganics: sonosynthesis.

Ultrasound irradiation covers many chemical reactions crucially aiming to design and synthesize various structured materials as an enduring trend in frontier research studies. Here, we focus on the latest progress of ultrasound-assisted synthesis and present the basic principles or mechanisms of sonosynthesis (or sonochemical synthesis) from ultrasound irradiation in a brand new way, including primary sonosynthesis, secondary sonosynthesis, and synergetic sonosynthesis. This current review describes in detail the various sonochemical synthesis strategies for nano-structured inorganic materials and the unique aspects of products including the size, morphology, structure, and properties.

Elevated systemic immune inflammation index level is associated with disease activity in ulcerative colitis patients.

Background: It has been confirmed that high Systemic immune-inflammation index (SII) levels usually indicate poor outcomes in various diseases, especially on malignancies. However, the clinical significance of the SII in ulcerative colitis (UC) patients is remain unclear. Therefore, the purpose of our paper is to analyze the levels of SII in UC patients and assess the relationship between the SII and disease activity.