Publications by authors named "Tingting Zhang"

EP400 encodes a core catalytic ATPase subunit of ATP-dependent chromatin remodeling complexes. The gene-disease association of EP400 is undetermined. In this study, we performed trio-based whole-exome sequencing in a cohort of 402 families with epilepsy and neurodevelopmental disorders (NDDs) and identified compound heterozygous EP400 variants in six unrelated individuals.

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Cathelicidins have anti-inflammatory activity and chicken cathelicidin-2 (CATH-2) has shown to modulate immune response, but the underlying mechanism of its anti-inflammation is still unclear. Therefore, in this study, we investigated the anti-inflammatory activity of CATH-2 on murine peritoneal macrophages during avian pathogenic (APEC) infection. The results showed that CATH-2 priming significantly reduced the production of IL-1β, IL-6, IL-1α, and IL-12.

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Targeted drug delivery systems exploiting the acidic tumor microenvironment have been thriving, with responsive modifications to the active sites like hydroxyl groups enhancing stability, reducing toxicity, and boosting targeted bioavailability. Yet, the utility of hydroxyl-based acid-sensitive linkages is currently constrained by the intricacy of synthesis, questionable stability, and an inability to discern between physiological pH levels associated with health and disease. Here, we unveiled a novel hydroxyl-based acid-labile linkage, the "acetal ester bond", which not only excelled in its hypersensitive acid responsiveness, but was also facile to synthesize, with exceptionally high yields (>80 %) of final products derived from primary, linear secondary, cyclic secondary, and tertiary alcohols consistently, demonstrating its broad applicability.

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Garlic is a widely utilized condiment and health product. However, garlic bulbs are prone to quality deterioration resulting in decrease of economic value during postharvest. In this study, the storability of 501 garlic accessions worldwide was evaluated based on the examination of decay index (DI), decay rate, sprouting rate, and bud-to-clove ratio in two consecutive years.

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Aberrant upregulation of hepatic lipogenesis induced by chronic and excessive alcohol consumption is a critical driver of the progression of alcohol-associated liver disease (ALD), however, no effective approaches inhibiting lipogenesis are currently available for treating ALD patients. Moreover, little is known about whether and how nonethanol ingredients in alcoholic beverages regulate the pathogenesis of ALD. Here the discovery of a small molecule that activates the production and secretion of fibroblast growth factor 21 (FGF21) is reported.

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Article Synopsis
  • * Researchers used light to activate specially designed nanoparticles that can increase the permeability of the BSCB, allowing normally restricted peptides to enter the spinal cord without harming the surrounding cells or affecting movement.
  • * The method effectively delivered the peptide bombesin into the spinal cord, leading to a quick rise in itching behavior in mice, showcasing potential for non-invasive therapies targeting spinal cord conditions.
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Effective subunit vaccines for tuberculosis (TB) must target antigenic components at various stages of infection. In this study, we constructed fusion proteins using secreted antigens from (), specifically ESAT6, CFP10, MPT64, and Rv2645 from the proliferation stage, along with latency-associated antigens Rv1738 and Rv1978. The resulting fusion proteins, designated LT33 (ESAT6-CFP10-Rv1738) and LT28 (MPT64-Rv1978-Rv2645), were combined with an adjuvant containing dimethyldioctadecylammonium bromide (DDA), polyriboinosinic polyribocytidylic acid (PolyI:C), and cholesterol to construct subunit vaccines.

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High concentrations of energetic compounds such as 2,4,6-trinitrotoluene (TNT), hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) in military-contaminated sites pose a serious threat to human health and ecosystems. Better understanding about their effects on microbial diversity and functional genes in soil of ammunition demolition sites is required. In this study, the information of soil microbial community composition was obtained by metagenomic sequencing, and the impacts of energetic compounds on microbial community structure at the level of functional genes and enzymes based on Nr (Non-Redundant Protein Sequence Database), KEGG (Kyoto Encyclopedia of Genes and Genomes), CAZy (Carbohydrate-Active enZymes Database) and other databases were discussed.

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Kv channels generate A-type current known to regulate neuronal excitability. Its role in processing timing information is understudied, especially in the auditory system where temporal information is crucial for hearing. In the cochlear nucleus, principal bushy neurons are specialized for temporal processing with distinct biophysical properties owing to their expression of various voltage-gated ion channels.

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Background And Purpose: The current therapy cannot meet the needs of glioblastoma (GBM). V-domain immunoglobulin suppressor of T-cell activation (VISTA) is significantly up-regulated in GBM patients; however, its therapeutic potential in GBM is still unclear.

Experimental Approach: Flow cytometry was used to detect the expression of VISTA and the co-expression pattern of VISTA and programmed death receptor 1 (PD-1) on brain infiltrating lymphocytes of GBM mice.

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GW117 functions as both an MT1/MT2 receptor agonist and a 5-HT2C receptor antagonist. This study aimed to investigate the anxiolytic effects of GW117 through behavioral assessments, including the open field test and novelty-suppressed feeding test (NSFT) within a chronic unpredictable mild stress (CUMS) model. GW117 was administered via oral gavage for 21 days to evaluate its sustained anxiolytic effects, with behavioral tests including the NSFT, the Vogel-conflict test, and the O-maze test.

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Article Synopsis
  • Placental accreta spectrum (PAS) with bladder involvement can lead to significant complications for both mothers and newborns, making accurate diagnosis essential for treatment planning.
  • This study aimed to compare the diagnostic accuracy of 2D ultrasound, 3D Crystal Vue technology, and MRI in identifying PAS cases that involve the bladder.
  • Results showed that specific imaging features, like bridging vessels and the obliteration of the tramline sign on Crystal Vue, were strong predictors of bladder involvement in PAS.
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To promote the application of freeze-thaw-dehydrated (FTD) potatoes and their gels, this study aimed to investigate the effects of freezing temperature on the physicochemical and gel properties of FTD potato powder and their correlation. The results revealed that, as the freezing temperature decreased, the solubility exhibited an overall downwards trend resulting from soluble solids and amylose liberation. Owing to the better cell integrity at -20 °C, the solubility was greater than that of the other treatment groups.

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A recently identified virus, chicken circovirus (ChCV), has been linked to the onset of acute gastroenteritis in chicks, a condition that can have a detrimental impact on the overall health and well-being of chickens in a farming setting. In this study, we developed an enzyme-linked immunosorbent assay (ELISA) method for the indirect detection of antibodies against the chicken circovirus (ChCV) through codon optimization, which effectively expressed the capsid protein of the ChCV and utilized it as an encapsulated antigen following purification. In establishing the ELISA method for detecting antibodies using the purified Cap protein as the antigen, the optimal concentration of the antigen was determined to be 1 µg/mL, the optimal blocking solution was identified as 1% bovine serum albumin, the optimal dilution ratio of the serum to be tested was established to be 1:100, and the dilution ratio of the secondary antibody was determined to be 1:5,000.

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Article Synopsis
  • The study aimed to evaluate the effectiveness of combining percutaneous kyphoplasty (PKP) with microwave ablation (MWA) for treating pain and improving outcomes in patients with spinal metastases.
  • A total of 87 patients were analyzed, showing that those who underwent the PKP + MWA treatment experienced better recovery in various health metrics compared to those who only had PKP.
  • The PKP + MWA group had improved pain relief, better quality of life, and higher survival rates within one year, while adverse events were similar between the two groups.
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Background: Most patients with hepatocellular carcinoma (HCC) have lost the opportunity for direct surgery at the time of diagnosis. Transarterial chemoembolization (TACE) combined with immune checkpoint inhibitors or tyrosine kinase inhibitors (TKI) can partially transform some unresectable HCC and improve the prognosis effectively. However, based on the promising prospects of combined targeted and immunotherapy for the effective treatment of HCC, the positive role of palliative surgery in the conversion treatment of advanced HCC urgently needs further intensive re-assessment.

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InP quantum dots (QDs) have emerged as promising nanomaterials in various fields due to their exceptional optical properties. However, its wide emission linewidth limits further application. In this study, we synthesized high-quality InP/ZnSe/ZnS QDs by suppressing hole defects.

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Background: Diarrhea and intestinal dysfunction commonly occur in young mammals, causing malnutrition and growth retardation in both human and livestock. As the traditional Chinese herb, mulberry leaf contains various bioactive compounds and showed several health benefits, such as regulating glucose and lipid metabolism, and modulating gut microbiota. Mulberry leaf exhibits the potential to modulate redox homeostasis and improve gut health, but the function and underlying mechanisms remains elucidative.

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The expression levels of small extracellular vesicles (sEVs) are closely associated with several significant biological processes, which can be used as a crucial biomarker for cancer diagnosis, such as colorectal cancer. More efforts are still necessary to amplify sEV detection sensitivity, as their expression is minimal during the early stages of colorectal cancer. Through the integration of a catalytic assembly-triggered DNAzyme motor and gold nanoparticle (AuNP) aggregation, we have developed a triple signal amplified biosensor for the detection of sEVs.

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Herein, we reported the development of a water-soluble conjugated polyelectrolyte (CPE) for efficient CO photoreduction under visible light ( > 420 nm). Bearing a fluorene-benzothiadiazole backbone and imidazolium-functionalized side chains, PFBT-Im exhibits remarkable photocatalytic activity with nearly 100% selectivity and a CO yield of 453.16 μmol g within 4 h, which is 8.

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Background: Excessive use of antibiotics is a widespread problem. We aim to evaluate the efficacy of a multifaceted intervention for reducing antibiotic use in patients with respiratory tract infections (RTIs).

Methods: In this two-arm cluster randomized controlled trial, we enrolled patients aged 18+ with symptomatic RTIs at 40 township health centers (THCs) selected from 10 counties in Anhui, China.

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Synopsis of recent research by authors named "Tingting Zhang"

  • - Tingting Zhang's recent research focuses on the development and application of novel nanoparticle technologies for improved detection and treatment methods in various health and environmental contexts, including sensitive detection of acetylcholine and organophosphorus pesticides using polysaccharide-stabilized palladium nanoparticles.
  • - Zhang is also investigating the ecological adaptations and germination processes of plant species, specifically in response to temperature and drought stress, contributing to the understanding of plant survival in changing environments.
  • - The author is actively studying the implications of self-medication with antibiotics in low- and middle-income countries, highlighting the need for improved public health strategies to combat antibiotic resistance, along with exploring the role of obesity-related metabolites in cancer progression and outcomes.