Characterization and prebiotic potential of polysaccharides from Rosa roxburghii Tratt pomace by ultrasound-assisted extraction.

In this study, polysaccharides (RRTPs) were extracted from Rosa roxburghii Tratt pomace by hot water or ultrasound (US)-assisted extraction. The structural properties and potential prebiotic functions of RRTPs were investigated. Structural characterization was conducted through HPAEC, HPGPC, GC-MS, FT-IR and SEM.

Endoglucanase H from Plays an Important Role in the Virulence on Pear Fruits.

can infect many agricultural products including cereals, grapes, and pear. Pathogenic fungi secrete diverse effectors as invasive weapons for successful invasion the host plant. During the pathogen-host interaction, 4486 differentially expressed genes were observed in with 2773 up-regulated and 1713 down-regulated, whereas 8456 differentially expressed genes were detected in pear fruits with 4777 up-regulated and 3679 down-regulated.

Chitosan inhibits Penicillium expansum possibly by binding to DNA and triggering apoptosis.

Chitosan is a natural polysaccharide that is abundant, biocompatible and exhibits effective antifungal activity against various pathogenic fungi. However, the potential intracellular targets of chitosan in pathogenic fungi and the way of activity of chitosan are far from well known. The present work demonstrated that chitosan could inhibit Penicillium expansum, the principal causal agent of postharvest blue mold decay on apple fruits, by binding to DNA and triggering apoptosis.

The Prognosis of Advanced Non-Small Cell Lung Cancer Patients with Precision-Targeted Therapy Guided by NGS Testing or Routine Testing.

Purpose: We aim to observe the potential survival benefits of driver gene-guided targeted therapy in advanced non-small cell lung cancer (NSCLC) patients compared to non-targeted therapy. Additionally, the study aims to assess whether Next-generation sequencing technology (NGS)-guided targeted therapy can provide survival advantages for advanced NSCLC patients compared to conventional Epidermal growth factor receptor ()/anaplastic lymphoma kinase () gene detection.

Methods: Clinical data, genetic testing results, and treatment information of 1663 advanced lung cancer patients diagnosed by pathology from January 2013 to June 2019 in Jiangsu University Affiliated Lianyungang Hospital were collected.

Expanded Carbon Nanotube Fiber at the Liquid-Air Interface for High-Performance Fiber-Based Supercapacitors and Electrochemical Sensors.

Carbon nanotube fibers (CNTFs) are widely utilized in flexible and wearable electronics due to their outstanding electrical and mechanical properties. However, the spinning process of CNTFs has limited the CNTs from exposure, leading to an ultralow usage efficiency of individual CNTs. Here, we propose an electrochemical expansion strategy of a single CNTF at the liquid-air interface, forming a macroscopic spindle-shaped CNTF (SS-CNTF) with an enlarged volume of up to 5000-fold upon the spindle.

Multi-Parameter Detection of Urine Based on Electropolymerized PANI: PSS/AuNPs/SPCE.

Urine analysis is widely used in clinical practice to indicate human heathy status and is important for diagnosing chronic kidney disease (CKD). Ammonium ions (NH), urea, and creatinine metabolites are main clinical indicators in urine analysis of CKD patients. In this paper, NH selective electrodes were prepared using electropolymerized polyaniline-polystyrene sulfonate (PANI: PSS), and urea- and creatinine-sensing electrodes were prepared by modifying urease and creatinine deiminase, respectively.

Transcriptome Analysis and Functional Characterization Reveal That Gene Contributes to the Virulence of on Apple Fruits.

is the causal agent of blue mold decay on apple fruits and is also known to be the major producer of patulin, a mycotoxin that represents serious hazard to human health. Several mechanisms have been suggested to explain the pathogenesis of in host plants. Secreted effector proteins are vital for the pathogenicity of many fungal pathogens through manipulating their hosts for efficient colonization.

Tissue-like Conductive TiC/Sodium Alginate Hybrid Hydrogel for Electrochemical Sensing.

Endowed with a soft and conductive feature, hydrogels have been widely used as interface materials in bioelectronics to fulfill mechanical matching and bidirectional exchange between electronic platforms and living samples. Despite their ionic conductivity, the lack of electron mobility has limited their further applications in biosensing, especially in the field of electrochemical sensing. Here, we propose a TiC/sodium alginate (SA) hybrid hydrogel with not only a tissue-like mechanical strength (down to 80 kPa) but also a combined exchange interface for ions and electrons, realizing both mechanical and electrical coupling toward biological tissues.

[Using Quantitative Computed Tomography to Study the Correlation Between Physical Composition and Grip Strength in Young People].

Objective: To study with quantitative computed tomography (QCT) the correlation between grip strength and physical composition and waist and hip circumferences in young people with different body mass indexes (BMIs).

Methods: A total of 1310 young people who came to West China Hospital, Sichuan University for physical checkups and underwent chest QCT at our hospital from April to July 2021 were included in the study. Their data were collected and their BMIs were calculated.

Offensive or amusing? The study on the influence of brand-to-brand teasing on consumer engagement behavioral intention based on social media.

With the development of social media, advertising has migrated from traditional media to social media. Marketers are increasingly using social media's brand pages to actively create humorous dialogue interactions with other brands for brand communication to achieve positive business outcomes. Especially brand-to-brand's aggressive humor dialogue can also be an effective brand communication strategy.

Characterization of Bacillus velezensis E2 with abilities to degrade ochratoxin A and biocontrol against Aspergillus westerdijkiae fc-1.

Ochratoxin A (OTA), primarily produced by the fungi belonging to the species of Aspergillus and Penicillium, is one of the most common mycotoxins found in cereals and fruits. In addition to resulting in huge economic losses, OTA contamination also poses considerable threat to human and livestock health. Microbial degradation of mycotoxins has been considered with great potential in mycotoxins decontamination.

CT-determined low skeletal muscle mass predicts worse overall survival of gastric cancer in patients with cachexia.

Background: There were controversies for the association between computed tomography (CT)-determined low skeletal muscle mass (SMM) and overall survival (OS) in gastric cancer (GC). In this study, we investigated whether cachexia could be a potential confounding variable for this issue.

Methods: We retrospectively collected the patients of GC in our institution between July 2016 and January 2021.

Functional Xeno Nucleic Acids for Biomedical Application.

Functional nucleic acids(FNAs) refer to a type of oligonucleotides with functions over the traditional genetic roles of nucleic acids, which have been widely applied in screening, sensing and imaging fields. However, the potential application of FNAs in biomedical field is still restricted by the unsatisfactory stability, biocompatibility, biodistribution and immunity of natural nucleic acids(DNA/RNA). Xeno nucleic acids(XNAs) are a kind of nucleic acid analogues with chemically modified sugar groups that possess improved biological properties, including improved biological stability, increased binding affinity, reduced immune responses, and enhanced cell penetration or tissue specificity.

Ag@Pyc Nanocapsules as Electrochemiluminescence Emitters for an Ultrasensitive Assay of the APE1 Activity.

Herein, Ag@pyrenecarboxaldehyde nanocapsules (Ag@Pyc nanocapsules) as emitters were prepared to construct an ultrasensitive electrochemiluminescence (ECL) biosensor for the detection of the human apurinic/apyrimidinic endonuclease1 (APE1) activity. Ag nanoparticles on the surface of Pyc nanocapsules as coreaction accelerators could significantly promote coreactant peroxydisulfate (SO) to generate massive reactive intermediates of sulfate radical anion (SO), which interacted with the Pyc nanocapsules to achieve a strong ECL response. In addition, with the aid of APE1-triggered 3D DNA machine, trace target could be converted into a large number of mimic targets (MTs), which were positively correlated with the activity of APE1.

Construction of Fast-Walking Tetrahedral DNA Walker with Four Arms for Sensitive Detection and Intracellular Imaging of Apurinic/Apyrimidinic Endonuclease 1.

Herein, a novel tetrahedral DNA walker with four arms was engineered to travel efficiently on the 3D-tracks via catalyzed hairpin assembly autonomously, realizing the sensitive detection and activity assessment as well as intracellular imaging of apurinic/apyrimidinic endonuclease 1 (APE1). In contrast to traditional DNA walkers, the tetrahedral DNA walker with the rigid 3D framework structure and nonplanar multi-sites walking arms endowed with high collision efficiency, showing a fast walking rate and high nuclease resistance. Impressively, the initial rate of the tetrahedral DNA walker with four arms was 4.

Associations between bone mineral density and chronic obstructive pulmonary disease.

Objective: To assess the relationship between chronic obstructive pulmonary disease (COPD) severity and bone mineral density (BMD) in the whole body and different body areas.

Methods: This retrospective, cross-sectional study included patients with COPD. Demographic and lung function data, COPD severity scales, BMD, and T scores were collected.

3D MXene/PEDOT:PSS Composite Aerogel with a Controllable Patterning Property for Highly Sensitive Wearable Physical Monitoring and Robotic Tactile Sensing.

MXene based composite conductive aerogels have been extensively investigated as sensitive materials for wearable pressure sensors owing to their effective 3D network microstructures and the excellent conductivity of MXene. In this work, we fabricated a 3D porous TiCT MXene/poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) composite aerogel (MPCA) with a controllable patterning property utilizing the Cu-assisted electrogelation method. The prepared composite aerogel can be assembled into pressure sensors for wearable physical monitoring and high-resolution sensor microarrays for robotic tactile sensing.

Pyrenecarboxaldehyde encapsulated porous TiO nanoreactors for monitoring cellular GSH levels.

Recently, ternary electrochemiluminescence (ECL) system has become a hot research topic due to its great potential for improving ECL efficiency by promoting the generation of intermediates. However, it is still a great challenge to increase the utilization rate of intermediates in a ternary ECL system. Herein, we propose a strategy to increase the utilization rate of intermediates by designing pyrenecarboxaldehyde (Pyc) encapsulated porous titania (pTiO) nanospheres (Pyc@pTiO) as ECL nanoreactors for an integrated ternary (luminophore/coreactant/co-reaction accelerator, Pyc/SO/TiO) ECL system construction.

Relationship between economic liberalization and intellectual property protection with regional innovation in China. A case study of Chinese provinces.

International openness can affect regional innovation through more export opportunities, enhanced import competition and the spillover effects of foreign direct investment. Many studies have been conducted based on different countries for capturing the determinants of regional innovation, but very little literature is available with contradictory findings for the case of China. Based on 19 years' panel data of 31 Chinese provinces, this paper analyzes the impact of international openness on regional innovation measured by the number of patent grants.

Treating autism spectrum disorder by intervening with gut microbiota.

Autism spectrum disorder (ASD) comprises a group of neurodevelopmental disorders with a high prevalence in childhood. The gut microbiota can affect human cognition and moods and has a strong correlation with ASD. Microbiota transplantation, including faecal microbiota transplantation (FMT), probiotics, breastfeeding, formula feeding, gluten-free and casein-free (GFCF) diet and ketogenic diet therapy, may provide satisfying effects for ASD and its related various symptoms.

An integrated and conductive hydrogel-paper patch for simultaneous sensing of Chemical-Electrophysiological signals.

Simultaneous monitoring of electrophysiological and biochemical signals is of great importance in healthcare and fitness management, while the fabrication of highly integrated and flexible devices is crucial to these applications. Herein, we devised a multifunctional and flexible hydrogel-paper patch (HPP) that was capable of simultaneously real-time monitoring of electrocardiogram (ECG) signal and biochemical signal (glucose content) in sweat during exercise. The self-assembly of the highly porous PEDOT:PSS hydrogel on paper fiber provided the HPP with good conductivity and hydrophilic wettability for efficient electron transmission and substance diffusion, thereby enabling it to serve as a low-impedance ECG electrode and a highly sensitive glucose sensor.

An integrated electrochemical POCT platform for ultrasensitive circRNA detection towards hepatocellular carcinoma diagnosis.

Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer-related death. Circ-CDYL, one of the circular RNAs (circRNAs), is recognized as an independent marker for HCC early diagnosis. Point-of-care testing (POCT) of circRNA is essential and in great demand for clinical applications.

[Effect of Ferric-carbon Micro-electrolysis on Greenhouse Gas Emissions from Constructed Wetlands].

As the problem of global warming becomes increasingly serious, the greenhouse gas (GHG) emission reduction measures of constructed wetlands (CWs) have drawn significant attention. Ferric-carbon micro-electrolysis exhibits an excellent effect on wastewater purification as well as the potential to reduce GHG emissions. Therefore, to explore the impact of ferric-carbon micro-electrolysis on GHG emissions from intermittent aeration constructed wetlands, four kinds of different wetlands with different fillers were constructed.