Colorimetric LAMP Based on One-Step Modified Filter Paper to Screen Human Papillomavirus (HPV)16/18 from Clinical Samples.

Cervical cancer is among the most common malignant tumors in women. The development of rapid screening techniques plays an important role in early screening for cancer treatment. We have developed an HPV screening method, which effectively combines the high-efficiency nucleic acid enrichment of chitosan-modified filter paper and the rapid visual detectability of colorimetric LAMP, along with the enhancement of the tolerance ability of the pH-sensitive LAMP reagent to acidic original samples, making the detection of HPV 16/18 easy to carry out and reliable, which is helpful for the epidemiological prevention and control strategies of HPV-induced cancer.

Synergistic effect of bimetal in isoreticular Zn-Cu-1,3,5-benzenetricarboxylate on room temperature gaseous sulfides removal.

Isoreticular bimetal M-Cu-BTC has considerable potential in improving the sulfides removal performance of Cu-BTC. Herein, three transition metals, namely, Zn, Ni and Co, were assessed to fabricate M-Cu-BTC, a desirable isoreticular bimetal. Results demonstrated the feasibility of using Zn to fabricate an isoreticular bimetallic Zn-Cu-BTC.

Effects of different sperm sources on the clinical outcomes of oocyte maturation cycles combined with intracytoplasmic sperm injection.

Objectives: To evaluate the embryonic developments and clinical outcomes of different sperm sources with cycles of intracytoplasmic sperm injection (ICSI) and maturation (IVM).

Methods: This retrospective study was approved by the hospital ethics committee and conducted in the hospital fertilization (IVF) clinic. From January 2005 to December 2018, 239 infertile couples underwent IVM-ICSI cycles and were divided into three groups according to different sperm sources.

Human serum albumin promotes self-renewal and expansion of umbilical cord blood CD34 hematopoietic stem/progenitor cells.

Background: We investigated the effect of human serum albumin (HSA) on human umbilical cord blood (UCB) CD34 hematopoietic stem/progenitor cells (HSPCs) cultured and transplanted .

Methods: Human umbilical cord blood mononuclear cells were obtained by density gradient centrifugation. CD34 cells were then sorted by CD34 conjugated magnetic microbeads.

Paiteling induces apoptosis of cervical cancer cells by down-regulation of the E6/E7-Pi3k/Akt pathway: A network pharmacology.

Ethnopharmacological Relevance: Human papillomavirus (HPV) infection is considered to be the main pathogen causing intraepithelial neoplasia. Paiteling (PTL) has been used to treat intraepithelial neoplasia caused by human papillomavirus (HPV) infection for more than 20 years in China, but its specific mechanism of action is not very clear, and further research is still needed.

Objective: This study designed a comprehensive strategy to study the pharmacological mechanism of paiteling in regulating cervical cancer cell apoptosis by integrating LC-MS/MS, network pharmacology and pharmacological experiments.

Tangnaikang Alleviates Hyperglycemia and Improves Gut Microbiota in Diabetic Mice.

Dysregulation of gut microbiota contributes to the development of type 2 diabetes. To investigate the antidiabetic effect of Tangnaikang and its regulation of gut microbiota in diabetic KKAy mice, a type 2 diabetes mouse model was established by feeding KKAy mice with a high-fat diet (HFD) for 2 weeks. The diabetic KKAy mice were treated with vehicle, Acarbose, or different doses of Tangnaikang once a day for 8 weeks.

Chromosome aneuploidy analysis in embryos derived from in vivo and in vitro matured human oocytes.

Background: In vitro oocyte maturation (IVM) is being increasingly approached in assisted reproductive technology (ART). This study aimed to evaluate the quality of embryos generated by in-vitro matured immature follicles, as a guideline for further clinical decision-making.

Methods: A total of 52 couples with normal karyotypes underwent in vitro fertilization, and 162 embryos were donated for genetic screening.

Urinary sediment microRNAs can be used as potential noninvasive biomarkers for diagnosis, reflecting the severity and prognosis of diabetic nephropathy.

Background: Patients with both diabetes mellitus (DM) and kidney disease could have diabetic nephropathy (DN) or non-diabetic renal disease (NDRD). IgA nephropathy (IgAN) and membranous nephropathy (MN) are the major types of NDRD. No ideal noninvasive diagnostic model exists for differentiating them.

Predictive model for acute respiratory distress syndrome events in ICU patients in China using machine learning algorithms: a secondary analysis of a cohort study.

Background: To develop a machine learning model for predicting acute respiratory distress syndrome (ARDS) events through commonly available parameters, including baseline characteristics and clinical and laboratory parameters.

Methods: A secondary analysis of a multi-centre prospective observational cohort study from five hospitals in Beijing, China, was conducted from January 1, 2011, to August 31, 2014. A total of 296 patients at risk for developing ARDS admitted to medical intensive care units (ICUs) were included.

[Quantitative effect of the expression level of key genes in naringenin synthesis on the accumulation level of target products].

Naringenin is a natural flavonoid compound with anti-inflammatory, anti-oxidation, anti-viral, anti-atherosclerosis and other pharmacological activities. It is also an important precursor of other flavonoid synthesis and with great value of application. At present, the production of flavonoids such as naringenin by microbial methods has a low yield due to imbalance of metabolic pathways, which greatly limits its industrial application.

MicroRNA-181 targets Yin Yang 1 expression and inhibits cervical cancer progression.

Dysregulated expression of microRNAs (miRNAs) has been observed in numerous types of human cancer, including cervical cancer (CC). The present study aimed to elucidate the expression and roles of miR‑181 in cervical cancer tissues and cells. HeLa cells with a stable overexpression of miR‑181 were generated and injected subcutaneously into the front legs of nude mice.

LncRNA-HOST2 regulates cell biological behaviors in epithelial ovarian cancer through a mechanism involving microRNA let-7b.

Recently, a large number of long non-coding RNAs (lncRNAs) have been reported in mammalian genomes and are evolutionarily conserved and presumably function in many biological events, especially in the pathogenesis of diverse human cancers. A lncRNA, named HOST2 (human ovarian cancer-specific transcript 2), was once reported to specifically be expressed at high level in human ovarian cancer. However, how HOST2 acts to regulate gene functions in ovarian carcinogenesis has remained enigmatic.

Retrospective analysis of 80 cases with uterine carcinosarcoma, leiomyosarcoma and endometrial stromal sarcoma in China, 1988-2007.

Objective: Uterine sarcomas are rare gynecological malignancies with poor prognosis and high mortality. We provides clinical information of uterine sarcoma patients at Changhai Hospital of Secondary Military Medical University in Shanghai, China, over a 20-year period.

Design And Methods: Satisfied the criteria for the study, a total of 80 female patients with uterine sarcomas were retrospectively evaluated.

Importance of spondin 1 and cellular retinoic acid binding protein 1 in the clinical diagnosis of ovarian cancer.

Background: Diagnosis of ovarian cancer is often delayed because of subtle symptoms and a lack of a specific, sensitive test useful for the general population. The majority of cases are diagnosed at late stages, after the tumor has metastasized and implanted on many other abdominal organs and cavity surfaces. A paucity of prognostic markers makes it difficult to define which tumors will act aggressively and shorten survival.