Bioactive daphnane diterpenes from the flower buds of (Thymelaeaceae).

Four diterpenes of the daphnane type were isolated from a methanol extract of the flower buds of , the two of them were new structures named genkwadanes J () and K (). Their structures were determined based on analysis of their 1D- and 2D-NMR, HRESIMS and ECD calculations. Among the isolates, the cytotoxicity was assessed the MTT method using the K562, MCF-7 and HeLa cancer cell lines, the positive control was taxol.

The Potential Protective Effect of Iridoid Glycosides Isolated From Seeds Against the Development of Immune Liver Injury in Mice.

The iridoid glycosides were extracted and separated from seeds, and the potential protective effect of seed extract on concanavalin A-induced immune liver injury in mice was studied. seeds were extracted by 95% ethanol reflux. Then, the iridoid glycosides were enriched by extraction refined through petroleum ether (60°C-90°C), ethyl acetate, and water-saturated n-butanol in sequence, so as to purify the n-butanol part ( seed's n-butanol extraction, OFSN) by macroporous resin.

Hawthorn Leaf Flavonoids Protect against Diabetes-Induced Cardiomyopathy in Rats via PKC- Signaling Pathway.

Objectives: DCM has become one of the main reasons of death in diabetic patients. In this study, we aimed to explore the hawthorn leaf flavonoids (HLF) protective effect against diabetes-induced cardiac injury and the underlying mechanisms in experimental rats.

Methods: Experimental diabetic model was induced by intraperitoneal injection of streptozotocin (STZ, 40 mg/kg) in rats after feeding with high-fat diet for 8 weeks.

Metabonomics study on Polygonum multiflorum induced liver toxicity in rats by GC-MS.

Polygonum multiflorum, a traditional Chinese medicinal herb, is widely used in liver and liver nourishing. Recent years, drug regulatory departments reported that Polygonum multiflorum caused serious adverse reaction in clinic, especially liver injury. In this study, we detected the changes in rat serum and liver tissue metabolites through gas chromatography-mass spectrometry (GC-MS).

Effects of Polygonum multiflorum on the activity of cytochrome P450 isoforms in rats.

Polygonum multiflorum is a traditional Chinese medicinal herb used in clinical medicine to nourish the liver and kidney. However, in recent years, there have been increased reports of clinical adverse reactions associated with Polygonum multiflorum preparations, especially due to liver injury. The cocktail method can be used to assess the influence of Polygonum multiflorum on the activity of cytochrome P450 (CYP450) isoforms CYP2B6, CYP2C19, CYP2C9, CYP1A2, CYP3A4, and CYP2D6, which were reflected by changes in pharmacokinetic parameters in six specific probe drugs: bupropion, omeprazole, tolbutamide, phenacetin, midazolam, and metoprolol.