An electrochemical biosensor for sensitive detection of live Salmonella in food via MXene amplified methylene blue signals and electrostatic immobilization of bacteriophages.

A novel bacteriophage-targeted electrochemical biosensor designed for accurate and quantitative detection of live Salmonella in food samples is presented. The biosensor is simply constructed by electrostatic immobilizing bacteriophages on MXene-nanostructured electrodes. MXene, renowned for its high surface area, biocompatibility, and conductivity, serves as an ideal platform for bacteriophage immobilization.

Photoelectrochemical/Colorimetric Dual-Mode Specific Detection of Based on the Enzymatic Reaction Triggered by Catalase from Lysed Bacteria.

() is abundant in nature and frequently leads to various health issues. Bacteriophages as obligate intracellular parasites of bacteria have the ability to specifically identify and infect , causing bacterial lysis and the release of endogenous catalase (CAT). The released CAT triggers the conversion of HO into O and HO, resulting in a notable decrease in UV absorption at 570 nm and a concurrent surge in photocurrent.