[Cerebrospinal Fluid β2-Microglobulin for Prognostic Evaluation of Patients with Central Nervous System Infiltration in Acute Lymphoblastic Leukemia].

Objective: To investigate the prognostic value of serum and cerebrospinal fluid β2-microglobulin (β2-MG) in acute lymphoblastic leukemia (ALL) with central nervous system invasion after chemotherapy.

Methods: 40 patients with leukemia who had been confirmed to have central nervous system infiltration were selected for treatment at the Second Affiliated Hospital of Chongqing Medical University from January 2015 to May 2017, and the serum levels of β2-MG and CSF-β2MG were dynamically monitored and performed statistical analysis.

Results: After chemotherapy, the changes in serum β2-MG were not statistically significant (P>0.

[Metabolic Characteristics of Leucocyte-deplated and Suspended RBC Produced by Using Lipid Whole Blood During Routine Storage].

Objective: To investigate the differences of metabolic pathways of leucocyte-deplated RBCs prepared by using lipid whole blood and nomal blood during routine storage so as to provide some reference for clinical blood use.

Methods: Twenty U whole blood from 20 donors, including 10 U lipid blood and 10 U normal whole blood, were selected for preparing leukodepleted red blood cells, red blood cells were taken from storage bags on day 0, 14 and 35, respectively. Metabolites in the red blood cells were analyzed, red blood cell metabolic extracts were detected by UPLC-MS/MS.

[Expression of BAG3 Gene in Acute Myeloid Leukemia and Its Prognostic Value].

Objective: To investigate the expression of BAG3 gene in acue myeloid leukemia (AML) and its prognostic value.

Methods: Real-time quantitative RT-PCR was used to detect the expression of BAG3 mRNA in 88 previously untreated AML patients. The corelation of BAG3 expression level with clinical characteristics and known prognostic markers of AML was analyzed.

Effect of osteoprotegerin in combination with interleukin-6 on inhibition of osteoclast differentiation.

Objective: To observe the effect of recombinant interleukin-6 (IL-6) and osteoprotegerin (OPG) on inhibiting bone absorption induced by receptor activator for nuclear factor-kB ligand (RANKL) in murine osteoclast precursor cells (OCPs) model.

Methods: RAW 264.7 cells were solely treated with 50 ng/ml RANKL for 1 day, and then they were divided into three groups: RANKL (control group), RANKL+IL-6 (IL-6 group) and RANKL+IL-6+OPG (combination group).

[Construction of lentivirus-mediated short hairpin RNA targeting human STAT3 gene].

Aim: To construct and identify a lentivirus-mediated short hairpin RNA (shRNA) targeting human signal transducer and activator of transcription 3 (STAT3) gene.

Methods: The shRNA chains targeting to human STAT3 gene were designed and synthesized, and then inserted into lentivirus expression vector pSicoR containing U6 promoter and green fluorescent protein (GFP) gene by gene recombination technique. The constructed recombinant plasmid pSicoR-STAT3-shRNA was identified by double restriction enzyme digestion and DNA sequencing, and then mixed with the 3rd generation of lentiviral packaging system and co-transfected to HEK293 cells using new generation of Roche X-tremeGENE HP DNA Transfection Reagent mediated transfection method.

A novel immobilization multienzyme glucose fluorescence capillary biosensor.

Based on the immobilization enzyme technology and the fluorescence capillary analysis method, the authors have developed a highly sensitive fluorescence reaction system and a novel immobilization multienzyme glucose fluorescence capillary biosensor for determining glucose contents. Reaction principle of the system is that under the catalysis of glucose oxidase (GOD) and horseradish peroxidase (HRP) immobilized on inner surface of a medical capillary, beta-D-glucose reacts with dissolved oxygen to form gluconic acid-delta-lactone and hydrogen peroxide, and then the latter reacts with l-tyrosine to produce a tyrosine dimer, which has maximal excitation and emission wavelengths at 320 nm and 410 nm, respectively. Fluorescence of the dimer is proportional to the concentration of the beta-D-glucose.

Study of serum argininosuccinate lyase determination for diagnosis of liver diseases.

The objectives of this research were to establish an automatic analysis method for the determination of serum argininosuccinate lyase (ASL) and to investigate the value of serum ASL test in the diagnosis of various liver disorders. According to the chemical reaction catalyzed by ASL, an enzyme-coupled reaction system was designed, and a methodology evaluation of this method was performed. A total of 291 patients with various liver diseases, 247 patients with nonliver disease and 32 healthy controls, were recruited, their serum levels of ASL and traditional hepatopathy markers, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and total bilirubin (TBil), were all determined, and their diagnostic values in liver diseases were analyzed and compared.

[Determination of serum argininosuccinate lyase in diagnosing liver diseases].

Objective: To investigate the value of the determination of the levels of serum argininosuccinate lyase (ASL) in diagnosing various liver diseases.

Methods: Two hundred and ninety-one patients with various liver diseases, 257 patients with non-liver disease, and 32 healthy controls were recruited for this study and their serum ASL, ALT, AST, GGT, LDH, ALP, and total bilirubin (TBil) levels were determined. Liver biopsies were performed on 31 patients with hepatopathy.