[Subtype and sequence analysis of the gag genes for HIV-1 strains isolated in Hubei province].

Objective: To investigate the characteristic of subtypes and genetic diversity of HIV-1 circulating in Hubei province and its molecular epidemiological linkages with regard to risk factors of viral transmission.

Methods: plasma samples of 80 diagnosed individuals was characterized. The gene fragments of gag were amplified by reverse transcriptase polymerase chain reaction (RT-PCR) and HIV-1 genotypes were determined based on the nucleotide sequences of gag region.

[Study on molecular epidemiology of people infected with human immunodeficiency virus-1 in Hubei province].

Objective: To study the distribution of human immunodeficiency virus-1 (HIV-1) genotypes in Hubei province.

Methods: Epidemiological survey was carried out to HIV-1 carriers who were identified in Hubei province. HIV-1 env V3-V4, gag P17/24 and the first exon of tat region were amplified by nested-polymerase chain reaction(nPCR) .