Immunomodulatory activity in vitro and in vivo of polysaccharide from Potentilla anserina.

The polysaccharide (PAP) from Potentilla anserina was evaluated for modulating effects by using mouse peritoneal macrophage and the immunosuppressed-model cyclophosphamide-induced. Phagocytotic and mononuclear phagocytic system function assays showed that PAP stimulated the phagocytosis of phagocyte. Splenocyte proliferation assay showed that PAP acted the effect combining Con A or LPS in splenocyte proliferation.

[Detection of anti-Trichinella spiralis antibody by indirect ELISA using recombinant protein as antigen].

Objective: To study the specificity, sensitivity of diagnostic antigen for Trichinella spiralis (T.s).

Methods: T668 recombinant protein from highly efficient expression of newborn larvae stage-specific gene of T.

[Cloning and sequence analysis of a novel stage-specific cDNA from adult Trichinella spiralis].

Objective: To clone a stage-specific novel cDNA from 5 day-old adult worm (AD5) of Trichinella spiralis.

Methods: The cDNA library of AD5 was screened by an AD5 stage-specific cDNA probe labeled with digoxigenin (DIG). The positive clones were sequenced and analysed.