[Study on diagnosis of atypical cerebral cysticercosis].

Objective: To analyze the characteristics of the results of serum immunological tests and brain CT image examinations of atypical cerebral cysticercosis patients, so as to provide the reference for improving the diagnosis of the disease.

Methods: Totally 446 suspected cerebral cysticercosis patients were chosen as the study objects, all of them were given experimental treatment with praziquantel, and then the patients with atypical cerebral cysticercosis were diagnosed according to the treatment effect and review results of brain CT or MRI. Meanwhile, all the 446 patients were tested for serum specific IgG and IgG4 antibodies and cysticercus circulating antigen (CAg) by ELISA, McAb ELISA and PEG-ELISA respectively, and the IHA test was also performed.

[Extraction Method of Malaria Parasite DNA from Preserved Positive Blood Smears].

Objective: To develop a method for DNA extraction from malaria parasites on preserved blood smears, to provide basis for research on malaria genetic traceability.

Methods: The improved DNA extraction kit (QIAamp DNA Mini Kit) was used to extract plasmodium DNA from 41 giemsa-stained blood smears, and the extraction was compared with that using the Chelex-100 and Na(2)HPO(4) methods. Nested PCR was used to amplify small subunit ribosomal RNA to identify Plasmodium parasite.

[Comparative study on EEG and neuroimaging of patients with cerebral cysticercosis before and after treatment].

Objective: To study the changes of cerebral function and pathological morphology before and after the antiparasitic treatment with albendazole and praziquantel in patients with cerebral cysticercosis.

Methods: The data of EEG and neuroimaging of 412 patients with cerebral cysticercosis were retrospectively analyzed.

Results: Before the treatment, the mild abnormality, moderate abnormality, and severe abnormality were observed in 40.

[Clinical analysis of 91 cases of imported falciparum malaria from Africa].

Objective: To explore the clinical characteristics, early diagnosis, and treatment of patients with imported falciparum malaria from Africa.

Methods: The clinical data of 91 imported falciparum malaria cases were analyzed by retrospective study.

Results: All the 91 cases had the history of mosquito bites.