Publications by authors named "Ting-Kuan Chu"
Article Synopsis
- Rare cell populations are hard to study with traditional single-cell RNA sequencing (scRNA-seq) because they often require pooling from different samples, which loses valuable origin and phenotype information.
- To tackle this issue, researchers have developed a technique called sample multiplexing, using unique sequence barcodes for each sample to analyze them simultaneously without losing data quality.
- The study focuses on mouse retinal ganglion cells, utilizing this multiplexing approach to improve accuracy in tracking cell types and gene expression variability, and also helps identify multiplets in samples that lack specific labeling.
Rare cell populations can be challenging to characterize using microfluidic single-cell RNA sequencing (scRNA-seq) platforms. Typically, the population of interest must be enriched and pooled from multiple biological specimens for efficient collection. However, these practices preclude the resolution of sample origin together with phenotypic data and are problematic in experiments in which biological or technical variation is expected to be high (e.
View Article and Find Full Text PDFAging is the major risk factor for cardiovascular disease, which is the leading cause of mortality worldwide among aging populations. is a prolongevity gene that mediates lifespan in mammals. Previously, our investigations revealed that a persistently high level of expression in mice is able to prevent age-associated cardiac dysfunction.
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