Synthesis, characterization and electrochemical properties of stable osmabenzenes containing PPh3 substituents.

Treatment of [OsCl(2)(PPh(3))(3)] with HC[triple bond]CCH(OH)C[triple bond]CH/PPh(3) produces the osmabenzene [Os{CHC(PPh(3))CHC(PPh(3))CH}Cl(2)(PPh(3))(2)][OH] (2), which is air stable in both solution and solid state. The key intermediate of the one-pot reaction, [OsCl(2){CH=C(PPh(3))CH(OH)C[triple bond]CH}(PPh(3))(2)] (3), and the related complex [Os(NCS)(2){CHC(PPh(3))CH(OH)C[triple bond]CH}(PPh(3))(2)] (7) have been isolated and characterized, further supporting the proposed mechanisms for the reaction. Reactions of 3 with PPh(3), NaI, and NaSCN give osmabenzene 2, iodo-substituted osmabenzene [Os{CHC(PPh(3))CHCICH}I(2)(PPh(3))(2)] (4), and thiocyanato-substituted osmabenzene [Os{CHC(PPh(3))CHC(SCN)CH}(NCS)(2)(PPh(3))(2)] (5) respectively.

Dichlorido(η-cyclo-octa-1,5-diene)bis-(triphenyl-phosphine)osmium(II).

The Os(II) atom in the title compound, [OsCl(2)(C(8)H(12))(C(18)H(15)P)(2)], is located on a crystallographic twofold axis and adopts a distorted octa-hedral coordination geometry. The two triphenyl-phosphine ligands are trans to each other, while the two chlorine ligands are cis-disposed. The coordination is completed by the cyclo-octa-diene (COD) ligand with bonding to the two olefin double bonds.

The tail pigmentation pattern of C57BL/6J mice affects nociception/pain quantification in the tail flick test.

The heat radiant tail flick test is commonly used to quantify nociception and pain levels. Likewise, the C57BL/6J strain of mice is frequently used in pain-related studies as transgenic mice are often backcrossed onto this background. C57BL/6J mice naturally develop non-pigmented patches of variable length on the distal part of the tail that could conceivably modify the response latency in tail flick assays.

Sinulaflexiolides A-K, cembrane-type diterpenoids from the chinese soft coral Sinularia flexibilis.

A bioassay-guided fractionation and chemical examination of the soft coral Sinularia flexibilis resulted in the isolation and characterization of sinulaflexiolides A-K (1-11), along with sinulariolone (12), 5-dehydrosinularolide (13), capillolide (14), sinulariolide (15), 5,8-epoxy-9-acetoxysinulariolide (16), flexibilide (17), dihydroflexibilide (18), and the enantiomer of 14-deoxycrassin (19). Their structures were determined on the basis of extensive spectroscopic (IR, MS, 2D NMR) data analysis and by comparison with spectroscopic data reported in the literature. Sinulaflexiolides D and E showed selective inhibitory activity against the gastric gland carcinoma cell line BGC-823 at 8.

[Integrity of lumbar facet joint and curative effect after a lumbar intervertebral disc operation].

Objective: To analyze the relation between the clinical outcome and the integrity of the facets after a lumbar operation,and to provide a reference for choosing operative method and clinical prognosis.

Methods: Forty-three patients with complete data underwent uni-segment discectomy were enrolled. There were 3 surgical interventions: open-window discectomy, full or semi-laminectomy.

Osmabenzenes from the reactions of a dicationic osmabenzyne complex.

Treatment of the osmabenzyne Os([triple bond]CC(SiMe(3))=C(Me)C(SiMe(3))=CH)Cl(2)(PPh(3))(2) (1) with 2,2'-bipyridine (bipy) and thallium triflate (TlOTf) produces the thermally stable dicationic osmabenzyne [Os([triple bond]CC(SiMe(3))=C(Me)C(SiMe(3))=CH)(bipy)(PPh(3))(2)](OTf)(2) (2). The dicationic osmabenzyne 2 reacts with ROH (R = H, Me) to give osmabenzene complexes [Os(=C(OR)CH=C(Me)C(SiMe(3))=CH)(bipy)(PPh(3))(2)]OTf, in which the metallabenzene ring deviates significantly from planarity. In contrast, reaction of the dicationic complex 2 with NaBH(4) produces a cyclopentadienyl complex, presumably through the osmabenzene intermediate [Os(=CHC(SiMe(3))=C(Me)C(SiMe(3))=CH)(bipy)(PPh(3))(2)]OTf.

Demonstration of lysosomal localization for the mammalian ependymin-related protein using classical approaches combined with a novel density shift method.

Most newly synthesized soluble lysosomal proteins are delivered to the lysosome via the mannose 6-phosphate (Man-6-P)-targeting pathway. The presence of the Man-6-P post-translational modification allows these proteins to be affinity-purified on immobilized Man-6-P receptors. This approach has formed the basis for a number of proteomic studies that identified multiple as yet uncharacterized Man-6-P glycoproteins that may represent new lysosomal proteins.

Syntheses of metallabenzynes from an allenylcarbene complex.

Treatment of the allenylcarbene complex OsCl2(=CPh-CH=C=CHPh)(PPh3)2 with (PPh3)AuCCR in the presence of HNEt3Cl in CH2Cl2 produces osmabenzynes Os(CC(R)=C(CH2Ph)CH=CPh)Cl2(PPh3)2.

Osmabenzenes from the reactions of HC[triple bond]CCH(OH)C[triple bond]CH with OsX2(PPh3)3 (X = Cl, Br).

Treatment of OsX2(PPh3)3 (X = Cl, Br) with HCCCH(OH)CCH in THF produces OsX2(CH=C(PPh3)CH(OH)CCH)(PPh3)2, which reacts with PPh3 to give osmabenzenes [Os(CHC(PPh3)CHC(PPh3)CH)X2(PPh3)2]+.

Protonation and bromination of an osmabenzyne: reactions leading to the formation of new metallabenzynes.

The reactivities of benzynes and metal-carbyne complexes are normally associated with the triple bond units. However, we have now found that electrophiles do not attack the formal osmium-carbon triple bond of osmabenzyne complex 1. Instead, 1 undergoes electrophilic substitution reactions-the typical reactions of aromatic systems.