Dynamic Change of Volatile Fatty Acid Derivatives (VFADs) and Their Related Genes Analysis during Innovative Black Tea Processing.

Volatile fatty acid derivatives (VFADs) play a significant role in contributing to flowery-fruity flavor black tea. Innovative black tea is typically crafted from aroma-intensive tea cultivars, such as Jinmudan, using defined production methodologies. In this study, the during-processing tea leaves of innovative black tea were applied as materials, and we selected a total of 45 VFADs, comprising 11 derived aldehydes, nine derived alcohols, and 25 derived esters.

Using a DNA mini-barcode within the ITS region to identify toxic Amanita in mushroom poisoning cases.

Mushroom poisoning contributes significantly to global foodborne diseases and related fatalities. Amanita mushrooms frequently cause such poisonings; however, identifying these toxic species is challenging due to the unavailability of fresh and intact samples. It is often necessary to analyze residues, vomitus, or stomach extracts to obtain DNA sequences for the identification of species responsible for causing food poisoning.

A Bibliometric and Visual Analysis of the Current Status and Trends of Forensic Mixed Stain Research.

Objectives: To explore the context and hotspot changes of forensic mixed stain research through bibliometric approach.

Methods: The literature of forensic mixed stain included in the core collection of Web of Science database from 2011 to 2022 were collected as the study object, and the annual publication number, countrie (region), institution, journal, keywords, etc. were bibliometrically and visually analyzed using the R-based Bibliometrix 1.

Pharmacological and mechanistic aspects of quercetin in osteoporosis.

Osteoporosis (OP) is a bone disease associated with increasing age. Currently, the most common medications used to treat OP are anabolic agents, anti-resorptive agents, and medications with other mechanisms of action. However, many of these medications have unfavorable adverse effects or are not intended for long-term use, potentially exerting a severe negative impact on a patient's life and career and placing a heavy burden on families and society.

Dual-mode luminescence anti-counterfeiting and white light emission of NaGdF:Ce,Eu,Tb/carbon dot hydrophilic nanocomposite ink.

NaGdF:Ce,Eu,Tb nanocrystals were successfully prepared by a one-step hydrothermal method with Ce ions as sensitizers, Eu and Tb ions as activators, and polyethylenimine (PEI) as surfactants. Color-adjustable fluorescence emission was achieved by the energy transfer effect between rare earth ions. Blue fluorescent carbon quantum dots (CDs) with a double UV response under 254 nm and 365 nm excitation were synthesized by a one-step hydrothermal method.

Acid-Promoted Cyclization Reaction of the Guanine Base with 1,1,3,3-Tetramethoxypropane: A Method for the Preparation of M dG and its Derivatives.

Despite the importance of nucleosides and nucleotides for drug discovery, only a few practical methods to prepare tricyclic nucleosides have been reported. Here, we describe a synthetic strategy for late-stage functionalization of nucleosides and nucleotides via chemo- and site-selective acid-promoted intermolecular cyclization. The nucleoside analogs with an additional ring were obtained in moderate-to-high yields, including some antiviral drugs (acyclovir, ganciclovir, and penciclovir) derivatives, endogenous fused ring nucleoside (M dG) and its derivatives, and nucleotide derivatives.

Synthesis of Nucleoside and Nucleotide Analogues by Cyclization of the Guanine Base with 1,1,3,3-Tetramethoxypropane.

3-(2-Deoxy-β-d-erythropentofuranosyl)pyrimido[1,2-]purin-10(3)-one (MdG) is an endogenous DNA adduct in bacterial and mammalian cells that could be explored as a biomarker for oxidative stress. Nonetheless, the lack of an efficient methodology for the preparation of MdG hampers the deep investigation of its biosynthesis and biorelevant processes. In this project, we aimed to address this issue by developing a highly efficient method to synthesize MdG and its analogues.

Angiotensin II is a crucial factor in retinal aneurysm formation.

Retinal arterial macroaneurysms are characterized by the acquired fusiform or saccular dilatations of the retinal artery. Angiotensin II (Ang II) is a major signal molecule of the renin-angiotensin system, which exerts a range of pathogenic actions that are relevant to retinal vascular abnormalities. We aimed to study the effect of Ang II on retinal vessels and explore its relationship with retinal aneurysmal disease.

Oct4 Regulates the Transition of Cancer Stem-Like Cells to Tumor Endothelial-Like Cells in Human Liver Cancer.

Octamer-binding transcription factor 4 (Oct4) has been recently implicated as a proangiogenic regulator in several induced pluripotent stem cells (iPSCs), however, its role in cancer stem-like cells (CSCs) remain unclear. We report here that Oct4 participates in tumor vasculogenesis in liver CSCs (LCSCs). We identify that LCSCs possess the potential of endothelial -differentiation under endothelial induction, present endothelial specific markers and their functions , and participate in neovasculogenesis .

Dynamics of ADH and related genes responsible for the transformation of C-aldehydes to C-alcohols during the postharvest process of oolong tea.

Aroma is an important index of tea quality. The volatile C-compounds formed from linoleic and linolenic acids in tea leaf lipids are essential components of tea. C-compounds are formed and transformed during the postharvest process of tea leaves.

DNA barcoding and mini-barcoding in authenticating processed animal-derived food: A case study involving the Chinese market.

This study used DNA barcoding and DNA mini-barcoding to test a variety of animal-derived food products sold in the Chinese market for potential mislabeling. Samples (52) including meat, poultry, and fish purchased from retail and online sources were examined. Regions of cytochrome C oxidase I (COI) gene (~650 bp) and 16S rRNA (~220 bp) were used as full- and mini-barcode markers, respectively.

Erythromycin Regulates Cigarette Smoke-Induced Proinflammatory Mediator Release Through Sirtuin 1-Nuclear Factor κB Axis in Macrophages and Mice Lungs.

Background: Macrolides have anti-inflammatory and antioxidative stress function, but their pharmacological regulation remains unclear. Sirtuin 1 (SIRT1) is redox-sensitive protein belongs to class III histone/protein deacetylases, SIRT1 regulates the acetylation/expression of nuclear factor κB (NF-κB) and is involved in the airway inflammation of chronic obstructive pulmonary disease.

Objectives: The present study was designed to examine the effects of erythromycin (EM) on the SIRT1-NF-κB axis and NF-κB-dependent proinflammatory cytokines.

Waterproof Narrow-Band Fluoride Red Phosphor KTiF:Mn via Facile Superhydrophobic Surface Modification.

With unique and efficient narrow-band red emission and broadband blue light absorption characteristics, Mn-activated fluoride red phosphors have gained increasing attention in warm white LEDs (WLEDs) and liquid crystal display (LCD) backlighting applications, whereas the intrinsic hygroscopic nature of these phosphors have inevitably limited their practical applications. Herein, a waterproof narrow-band fluoride phosphor KTiF:Mn (KTF) has been demonstrated via a facile superhydrophobic surface-modification strategy. With the use of superhydrophobic surface modification with octadecyltrimethoxysilane (ODTMS) on KTF surfaces, the moisture-resistance performance and thermal stability of the phosphor KTF can be significantly improved.

Centrosymmetric (Hdima)[GeBO(OH)] and Noncentrosymmetric NaGaBO(OH): Solvothermal/Surfactant-Thermal Synthesis of Open-Framework Borogermanate and Galloborate.

Under solvothermal/surfactant-thermal conditions, two new open-framework borogermanate and galloborate, centrosymmetric (Hdima)[GeBO(OH)] (1; dima = dimethylamine) and noncentrosymmetric NaGaBO(OH) (2), were obtained and characterized. Compound 1 contains an unusual basket-shaped GeBO(OH) cluster and displays a 3D open-framework layered structure. Compound 2 shows an interrupted 3,4-connected network constructed by alternately linked BO units and Ga ions and displays weak second-harmonic-generation response.

Dendritic cells induce Tc1 cell differentiation via the CD40/CD40L pathway in mice after exposure to cigarette smoke.

Dendritic cells and CD8(+) T cells participate in the pathology of chronic obstructive pulmonary disease, including emphysema, but little is known of the involvement of the CD40/CD40L pathway. We investigated the role of the CD40/CD40L pathway in Tc1 cell differentiation induced by dendritic cells in a mouse model of emphysema, and in vitro. C57BL/6J wild-type and CD40(-/-) mice were exposed to cigarette smoke (CS) or not (control), for 24 wk.

Comparative profiling of gene expression in Camellia sinensis L. cultivar AnJiBaiCha leaves during periodic albinism.

The AnJiBaiCha albino mutant tea cultivar has a reversible albino phenotype at low temperatures. Albino AnJiBaiCha leaves contain high levels of amino acids, which are important components affecting the quality of tea as a beverage. To examine the molecular mechanism of albinism and amino acid enrichment in AnJiBaiCha, we used the amplified fragment length polymorphism (cDNA-AFLP) technique to isolate genes that are differentially expressed during periodic albinism in AnJiBaiCha.

[Effect and mechanisms of wenyang yiqi huoxue recipe on the apoptosis of photoreceptor cells in retinal degeneration slow mice].

Objective: To observe the effect of Wenyang Yiqi Huoxue Recipe (WYHR) on the apoptosis of photoreceptor cells in retinal degeneration slow (RDS) mice, and to investigate its molecular mechanisms.

Methods: RDS mice were randomly divided into the model group and the Chinese medicine group,and C57BL/6J mice were selected as the normal control group. Each group consisted of 4 female mice and 2 male mice.

Facile assembly of an unusual lead borate with different cluster building units via a hydrothermal process.

Pb(BO2)2·H2O as sources of B and Pb via a simple hydrothermal process provided the first binodal 5,9-connected lead borate, Pb6B4O11(OH)2 (1). Compound 1 crystallizes in the orthorhombic space group Pnma. The crystal structure is composed of different cluster building units of B4O9 and Pb6O4.