Reduced peroxisome proliferator-activated receptor-α and bile acid nuclear receptor NR1H4/FXR may affect the hepatic immune microenvironment of biliary atresia.

Background: Biliary atresia (BA) is a childhood liver disease characterized by fibrous obstruction and obstruction of the extrahepatic biliary system and is one of the most common and serious biliary disorders in infants. Significant inflammation and fibrosis of the liver and biliary tract are the most prominent features, regardless of the initial damage to the BA. Abnormalities in innate or adaptive immunity have been found in human patients and mouse models of BA.

Zinc insulin hexamer loaded alginate zinc hydrogel: Preparation, characterization and in vivo hypoglycemic ability.

Alginate zinc hydrogel loaded with zinc insulin hexamer was prepared and characterized for oral insulin administration. The hydrogel was fabricated by dripping zinc insulin hexamer into sodium alginate solution and followed by crosslinking by zinc chloride. SEM image reveals the zinc insulin hexamer was integrated into the matrix of hydrogel.

A new method for studying the mechanism of "Feature Identification based quality assessment" of Traditional Chinese Medicine, taking as an example.

Aim Of The Study: The research group proposed that the mechanism of "Feature Identification based Quality Assessment" (FIQA) of Traditional Chinese Medicine (TCM) can be explained according to the relationship between the "Feature" of TCM and the Pharmacodynamic Components representing the holistic effect of TCM. (GR) was selected as the research object to reveal the close relationship between "Feature" and the quality of TCM.

Materials And Methods: In this study, the "Feature" such as "Shape", "Color", "Odour" and "Taste" of GR are quantified by the electronic nose, electronic tongue, and other instruments.

Temperature and pH levels: Key factors effecting hidden/free zearalenone during maize processing.

The productions of food, agricultural products and feed involve a variety processing conditions, which can affect the release/binding of zearalenone (ZEN) with macro-components in maize, resulting in the interconversion of hidden ZEN with free ZEN. This interconversion can cause the uncertainties and individual differences in the exposure assessment. To fully overcome this uncertainties of the potential risks for humans and animals, further research regarding the key factors and rule of interconversion is needed.

Altered gray matter volume and functional connectivity in medial orbitofrontal cortex of bulimia nervosa patients: A combined VBM and FC study.

Brain structural and functional abnormalities have been shown to be involved in the neurobiological underpinnings of bulimia nervosa (BN), while the mechanisms underlying this dysregulation are unclear. The main goal of this investigation was to explore the presence of brain structural alterations and relevant functional changes in BN. We hypothesized that BN patients had regional gray matter volume abnormalities and corresponding resting-state functional connectivity (rsFC) changes compared with healthy controls.

Clamping Fatigue Properties of Shrink-Fit Holder.

In order to explore the clamping fatigue properties of shrink-fit holders, ANSYS software was used in this study to analyze the thermal and contact stresses during the clamping process of the shrink-fit holder, and the fatigue analysis was performed by selecting the dangerous areas based on the two stresses. A numerical control shrink-fit holder clamping fatigue test device was manufactured, and the automatic clamping of the shrink-fit holder was executed in this study. After 500 clamping repetitions, a milling test was carried out on the shrink-fit bracket.

lncRNA WT1-AS attenuates hypoxia/ischemia-induced neuronal injury during cerebral ischemic stroke via miR-186-5p/XIAP axis.

This study aimed to investigate the role and mechanism of long non-coding RNA (lncRNA) WT1 antisense RNA (WT1-AS) in cerebral ischemic stroke. The Starbase database and dual-luciferase reporter gene assay were used to analyze the interaction between lncRNA WT1 antisense RNA (lncRNA WT1-AS) and microRNA-186-5p (miR-186-5p). Reverse transcription-quantitative PCR analysis was performed to determine lncRNA WT1-AS and miR-186-5p levels.

Clinical significance and interrelations of PD-L1 expression, Ki-67 index, and molecular alterations in sporadic medullary thyroid carcinoma from a Chinese population.

Immunotherapy shows prospects in treating advanced medullary thyroid carcinoma although controversial reports are present. Recently, histological grading has been applied to medullary thyroid carcinoma by the Ki-67 index, mitotic figures, and tumor necrosis. However, the interrelation of PD-L1 expression, the Ki-67 index, and major genetic alterations of sporadic medullary thyroid carcinoma has not been fully reported.

Artificial intelligence using deep neural network learning for automatic location of the interscalene brachial plexus in ultrasound images.

Background: Identifying the interscalene brachial plexus can be challenging during ultrasound-guided interscalene block.

Objective: We hypothesised that an algorithm based on deep learning could locate the interscalene brachial plexus in ultrasound images better than a nonexpert anaesthesiologist, thus possessing the potential to aid anaesthesiologists.

Design: Observational study.

Case Report: Variants Cause Primary Ciliary Dyskinesia and Infertility in Two Han Chinese Families.

Primary ciliary dyskinesia (PCD) is a rare genetic disorder, predominantly autosomal recessive. The dynein axonemal assembly factor 4 () is mainly involved in the preassembly of multisubunit dynein protein, which is fundamental to the proper functioning of cilia and flagella. There are few reports of PCD-related pathogenic variants of , and almost no -related articles focused on sperm phenotype.

Advances in MUC1-Mediated Breast Cancer Immunotherapy.

Breast cancer (BRCA) is the leading cause of death from malignant tumors among women. Fortunately, however, immunotherapy has recently become a prospective BRCA treatment with encouraging achievements and mild safety profiles. Since the overexpression and aberrant glycosylation of MUC1 (human mucin) are closely associated with BRCA, it has become an ideal target for BRCA immunotherapies.

Case Report: Whole-Exome Sequencing-Based Copy Number Variation Analysis Identified a Novel Homozygous Exon Deletion in a Patient With Primary Ciliary Dyskinesia.

Whole-exome sequencing (WES) based copy number variation (CNV) analysis has been reported to improve the diagnostic rate in rare genetic diseases. In this study, we aim to find the disease-associated variants in a highly suspected primary ciliary dyskinesia (PCD) patient without a genetic diagnosis by routine WES analysis. We identified the CNVs using the "Exomedepth" package in an undiagnosed PCD patient with a negative result through routine WES analysis.

Cyclovirobuxine D Ameliorates Experimental Diabetic Cardiomyopathy by Inhibiting Cardiomyocyte Pyroptosis NLRP3 and .

Diabetic cardiomyopathy (DCM) is one of the common complications of diabetic patients, which can induce myocardial hypertrophy, cardiac fibrosis, and heart failure. Growing evidence has shown that the occurrence and development of DCM are accompanied by pyroptosis which is an NLRP3-mediated intense inflammatory cell death. Cyclovirobuxine D (CVB-D) has been shown to significantly ameliorate DCM and anti-inflammatory effects associated with cardiomyopathy, but it is unclear whether it has an effect on cardiomyocyte pyroptosis accompanying DCM.

Identification of a Novel Variant in a Patient with Primary Ciliary Dyskinesia.

Background: encodes a protein with 1012 amino acids, which is a component of basal bodies and centrioles, essential for cilia biogenesis. was reported to be associated with X-chromosome linked dysmorphology syndrome in early studies and recent studies reported a few cases with primary ciliary dyskinesia (PCD) caused by deficiency.

Case Presentation: We report a 31-year-old man who suffered from recurrent respiratory infections with typical manifestations of primary ciliary dyskinesia.

The significance of thermomechanical processing on the cellular response of biomedical Co-Cr-Mo alloys.

Strengthening of biomedical Co-Cr-Mo alloys has been explored via thermomechanical processing for enhancing the durability of their biomedical applications. However, the effects of cold and hot deformation on the cellular activity continue to be unclear. In this study, we prepared Co-Cr-Mo alloy rods via cold swaging and hot-caliber rolling and studied the relationship between the microstructure and cellular response of pre-osteoblasts.

MRI based radiomics enhances prediction of neurodevelopmental outcome in very preterm neonates.

To predict adverse neurodevelopmental outcome of very preterm neonates. A total of 166 preterm neonates born between 24-32 weeks' gestation underwent brain MRI early in life. Radiomics features were extracted from T1- and T2- weighted images.

Novel Variants Associated With Primary Ciliary Dyskinesia-Related Infertility in Three Chinese Families.

The radial spoke head component 4A () is involved in the assembly of radial spokes, which is essential for motile cilia function. Asthenoteratozoospermia in primary ciliary dyskinesia (PCD) related to variants has not been reported. variants were identified and validated using whole-exome and Sanger sequencing in three unrelated Chinese families.

Icariside II suppresses the tumorigenesis and development of ovarian cancer by regulating miR-144-3p/IGF2R axis.

Ovarian cancer is one of the three major gynecological malignancies. It has been reported that Icariside II was able to block the occurrence and development of ovarian cancer. However, the detailed mechanism by which Icariside II regulates the development of ovarian cancer is widely unknown.

Extracellular Matrix Stiffness in Lung Health and Disease.

The extracellular matrix (ECM) provides structural support and imparts a wide variety of environmental cues to cells. In the past decade, a growing body of work revealed that the mechanical properties of the ECM, commonly known as matrix stiffness, regulate the fundamental cellular processes of the lung. There is growing appreciation that mechanical interplays between cells and associated ECM are essential to maintain lung homeostasis.

Neonatal pain, thalamic development and sensory processing behaviour in children born very preterm.

Background: Altered sensory processing is commonly reported in children born very preterm (≤32 weeks' gestational age [GA]). The immature nervous system, particularly the development of connections from the thalamus to the cortex, may show enhanced vulnerability to excessive sensory stimulation, and may contribute to altered sensory processing. Our objective was to determine whether sensory processing assessed at preschool-aged in children born very preterm was predicted by neonatal procedural pain and thalamic development.

Homozygous Variant in KASH5 Causes Premature Ovarian Insufficiency by Disordered Meiotic Homologous Pairing.

Context: Premature ovarian insufficiency (POI) affects 1% to 3.7% of women at reproductive age, and its etiology is heterogeneous. The linker of nucleoskeleton and cytoskeleton (LINC) complex, consisting of KASH5 and SUN1, plays an indispensable role in meiotic homolog pairing, determining the ovarian reserve.

Effects of amino acid composition of yeast extract on the microbiota and aroma quality of fermented soy sauce.

Yeast extracts, of which amino acids are the main component, can be directly applied to improve the flavor of final soy sauce. In this study, the potential of commercial yeast extracts was explored from amino acid approach to enhance the flavor quality of soy sauce by shaping the core fermentation microbiota. Alkaline and neutral amino acids favored the competitive benefits of flavor-producing bacteria, while acidic amino acids promoted the stress resistance of the fermentation microbiota, especially the abundance of Lactobacillus, which increased to 18.

Fluorescence sensor based on molecularly imprinted polymers and core-shell upconversion nanoparticles@metal-organic frameworks for detection of bovine serum albumin.

A novel strategy for sensing protein was proposed through combining the high selectivity of molecular imprinting technology with the excellent upconversion fluorescence of upconversion nanoparticles (UCNPs) and high specific surface area of metal-organic frameworks (MOFs). Herein, the UCNPs acted as signal reporter and MOFs were introduced to increase the rate of mass transfer. The UCNPs@MIL-100 as support material was prepared via a step-by-step method.

Evaluation of the Emotion Model in Electronic Music Based on PSO-BP.

Electronic music can help people alleviate the pressure in life and work. It is a way to express people's emotional needs. With the increase of the types and quantity of electronic music, the traditional electronic music classification and emotional analysis cannot meet people's more and more detailed emotional needs.

P2X2 receptors in pyramidal neurons are critical for regulating vulnerability to chronic stress.

Stress is a major risk factor for the development of depression. However, the underlying molecular mechanisms of stress vulnerability in depression are largely uncharacterized. P2X2 receptors (a major receptor for gliotransmitter-ATP) in the medial prefrontal cortex (mPFC) were identified by real-time qPCR, western blots and RNAscope hybridization in chronic social defeat stress model (CSDS).