Publications by authors named "Tinatini Tavhelidse-Suck"
Article Synopsis
- Medaka fish serve as an important model for exploring the genetics of vertebrate vision, using a novel optomotor response (OMR) assay to assess visual functions such as acuity and contrast sensitivity.
- The OMR assay features adjustable moving stripes displayed on a tablet, allowing for the testing of visual responses under various conditions, revealing significant strain-specific differences among medaka inbred strains.
- This efficient and open-source setup enables detailed quantitative mapping of visual traits and the underlying genetic mechanisms, with potential applications in studying various aspects of visual processing and neuron trainability.
CRISPR/Cas-based approaches have largely replaced conventional gene targeting strategies. However, homology-directed repair (HDR) in the mouse genome is not very efficient, and precisely inserting longer sequences using HDR remains challenging given that donor constructs preferentially integrate as concatemers. Here, we showed that injecting 5' biotinylated donor DNA into mouse embryos at the two-cell stage led to efficient single-copy HDR (scHDR) allele generation.
View Article and Find Full Text PDFPrecise, targeted genome editing by CRISPR/Cas9 is key for basic research and translational approaches in model and non-model systems. While active in all species tested so far, editing efficiencies still leave room for improvement. The bacterial Cas9 needs to be efficiently shuttled into the nucleus as attempted by fusion with nuclear localization signals (NLSs).
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