Lipid labeling from acetate or glycerol in cultured embryos of Camelina sativa seeds: A tale of two substrates.

Studies on the metabolism of lipids in seeds frequently use radiolabeled acetate and glycerol supplied to excised developing seeds to track the biosynthesis of acyl and lipid head groups, respectively. Such experiments are generally restricted to shorter time periods and the results may not quantitatively reflect in planta rates. These limitations can be removed by using cultured embryos, provided they mimic growth and lipid deposition observed for embryos in planta.

Lipid analysis of developing Camelina sativa seeds and cultured embryos.

Camelina sativa is a cultivated oilseed rich in triacylglycerols containing oleic, linoleic, α-linolenic and eicosenoic acids. As it holds promise as a model species, its lipid synthesis was characterized in vivo and in culture. Lipid accumulates at a maximum rate of about 26 μg/day/seed (11.

Effects of student participation in hand hygiene monitoring on knowledge and perception of infection control practices.

Studies report that students in health care professions do not retain knowledge of infection control and demonstrate poor hand hygiene compliance. This study describes the effect of a multifaceted approach (education, skills training, and monitoring) on nursing students' knowledge of infection control principles, opinions, hand hygiene practices, and value of nursing research in evidence-based practice. Students participated in hand hygiene monitoring of health care workers with 900 observations.

Comprehensive compositional analysis of plant cell walls (lignocellulosic biomass) part II: carbohydrates.

The need for renewable, carbon neutral, and sustainable raw materials for industry and society has become one of the most pressing issues for the 21st century. This has rekindled interest in the use of plant products as industrial raw materials for the production of liquid fuels for transportation(2) and other products such as biocomposite materials(6). Plant biomass remains one of the greatest untapped reserves on the planet(4).

Comprehensive compositional analysis of plant cell walls (Lignocellulosic biomass) part I: lignin.

The need for renewable, carbon neutral, and sustainable raw materials for industry and society has become one of the most pressing issues for the 21st century. This has rekindled interest in the use of plant products as industrial raw materials for the production of liquid fuels for transportation(1) and other products such as biocomposite materials(7). Plant biomass remains one of the greatest untapped reserves on the planet(4).

Preferences for teaching methods in a baccalaureate nursing program: how second-degree and traditional students differ.

Accelerated baccalaureate nursing programs are in great demand in the United States. Currently there are 197 such programs, but little research has been conducted on student characteristics and program outcomes. This quantitative study explores preferences of second-degree students and traditional generic students with regard to teaching methods and relationships with faculty.

The effect of conventional and sustained delivery of thymoquinone and levodopa on SH-SY5Y human neuroblastoma cells.

The objective of this study was to determine the effects of thymoquinone on the viability and metabolic activity of SH-SY5Y human neuroblastoma cells alone or challenged with levodopa (L-dopa) using conventional and sustained drug delivery routes. The findings suggest that thymoquinone may protect SH-SY5Y cells against L-dopa toxicity to some degree although the exact mechanism for this is unknown. Thymoquinone may be a viable option to prevent quinone formation as a result of L-dopa auto-oxidation and could be investigated as a neuroprotective agent given the fact that inflammatory etiologies are strongly implemented in the pathogenesis of Parkinson's disease.

The effect of conventional and sustained delivery of levodopa on SH-SY5Y neuroblastoma cells.

Unlabelled: L-dihydroxyphenylalanine-(L-dopa) has been used for the treatment of Parkinson's disease for decades. Recently it has been shown that L-dopa, at concentrations of 0.25 x 10(4) M or larger, can be toxic for human neuroblastoma cells.