Invasion of the body snatchers: the role of parasite introduction in host distribution and response to salinity in invaded estuaries.

In dynamic systems, organisms are faced with variable selective forces that may impose trade-offs. In estuaries, salinity is a strong driver of organismal diversity, while parasites shape species distributions and demography. We tested for trade-offs between low-salinity stress and parasitism in an invasive castrating parasite and its mud crab host along salinity gradients of two North Carolina rivers.

Efficient actuation design for optomechanical sensors.

For any nanomechanical device intended for sensing applications, actuation is an important consideration. Many different actuation mechanisms have been used, including self-oscillation, piezoelectric shakers, capacitive excitation, and optically pumping via the optical gradient force. Despite the relatively frequent use of optical pumping, the limits of optical actuation with a pump laser have not been fully explored.

Quantifying the effectiveness of shoreline armoring removal on coastal biota of Puget Sound.

Shoreline armoring is prevalent around the world with unprecedented human population growth and urbanization along coastal habitats. Armoring structures, such as riprap and bulkheads, that are built to prevent beach erosion and protect coastal infrastructure from storms and flooding can cause deterioration of habitats for migratory fish species, disrupt aquatic-terrestrial connectivity, and reduce overall coastal ecosystem health. Relative to armored shorelines, natural shorelines retain valuable habitats for macroinvertebrates and other coastal biota.

A methodological approach to scaling up fermentation and primary recovery processes to the manufacturing scale for vaccine production.

As the fermentation process is scaled up, the mass-transfer and nutritional requirements are to be maintained in improving antigen yields upon scale-up. A methodology is adopted to scale-down the processes at the laboratory level in order to further optimize the hydrodynamic and nutritional requirements of the bacteria prior to scaling up. For recovery of bacterial proteins, several micro-filtration techniques are discussed.

Mechanical stability of immobilized biocatalysts (CLECs) in dilute agitated suspensions.

Cross-linked enzyme crystals (CLECs) are a novel form of immobilized biocatalyst designed for application in industrial biotransformation processes. In this work we have investigated the mechanical stability of agitated CLEC suspensions in relation to the design and scale-up of bioconversions carried out in stirred-tank reactors. By careful control of the crystallization conditions yeast alcohol dehydrogenase I (YADHI) microcrystals of different size were first prepared having either an hexagonal (approximately 12 microm) or rod-shaped (approximately 4.