The nsp1, nsp13, and M proteins contribute to the hepatotropism of murine coronavirus JHM.WU.

Unlabelled: Mouse hepatitis virus (MHV) isolates JHM.WU and JHM.SD promote severe central nervous system disease.

The transcriptional co-regulator HCF-1 is required for INS-1 β-cell glucose-stimulated insulin secretion.

The transcriptional co-regulator host cell factor-1 (HCF-1) plays critical roles in promoting cell cycle progression in diverse cell types, and in maintaining self-renewal of embryonic stem cells, but its role in pancreatic β-cell function has not been investigated. Immunhistochemistry of mouse pancreas revealed nuclear expression of HCF-1 in pancreatic islets. Reducing HCF-1 expression in the INS-1 pancreatic β-cell line resulted in reduced cell proliferation, reduced glucose-stimulated insulin secretion, and reduced expression of the critical β-cell transcription factor Pdx1.

Murine coronavirus neuropathogenesis: determinants of virulence.

Murine coronavirus, mouse hepatitis virus (MHV), causes various diseases depending on the strain and route of inoculation. Both the JHM and A59 strains, when inoculated intracranially or intranasally, are neurovirulent. Comparison of the highly virulent JHM isolate, JHM.

The murine coronavirus nucleocapsid gene is a determinant of virulence.

The murine coronavirus, mouse hepatitis virus (MHV) strain A59, causes acute encephalitis and chronic demyelinating disease as well as hepatitis in mice. The JHM strain (also called MHV-4 or JHM.SD) causes fatal encephalitis and only minimal hepatitis.