Publications by authors named "Timothy B Brenneman"

Article Synopsis
  • Multiple field trials assessed the effectiveness of various demethylation-inhibiting (DMI) fungicides against pecan scab, revealing high resistance levels to tebuconazole in fungal isolates from different locations.
  • Genetic analysis identified specific mutations in resistance-related genes, including G444D, G357H, and I77T/I77L in resistant isolates compared to sensitive ones.
  • Enhanced expression of resistance-related genes in resistant isolates suggests that both point mutations and gene overexpression contribute to the observed resistance to tebuconazole.
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In Georgia, pecans are commercially grown in the Piedmont and Coastal Plain ecoregions which are characterized by sandy-loam, sandy, and/or clay soils. If well-drained, these soils are suitable for pecan production, but the soil characteristics differ enough between ecoregions in which the plant-parasitic nematode (PPN) communities could differ substantially. We studied PPN communities in pecan orchards to evaluate the potential for ecoregion differences.

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species are endophytes and pathogens of woody hosts and members of the Botryosphaeriaceae. Leaf dieback is a new disease resulting in death of compound leaves and extensive defoliation of pecan trees () throughout the southeastern United States. Currently, the disease is consistently most severe on trees that are not managed with fungicides for pecan scab.

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In peanut () production, in-furrow applications of the premix combination of the succinate-dehydrogenase-inhibitor (SDHI) fungicide and nematicide fluopyram and the insecticide imidacloprid are used primarily for management of nematode pests and for preventing feeding damage on foliage caused by tobacco thrips (). Fluopyram is also active against many fungal pathogens. However, the effect of in-furrow applications of fluopyram on early leaf spot () or late leaf spot () has not been characterized.

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Previous research has demonstrated the efficacy of prescription fungicide programs, based upon Peanut Rx, to reduce combined effects of early leaf spot (ELS), caused by (), and late leaf spot (LLS), caused by (syn. ), but the potential of Peanut Rx to predict each disease has never been formally evaluated. From 2010 to 2016, non-fungicide-treated peanut plots in Georgia and Florida were sampled to monitor the development of ELS and LLS.

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Sensitivity monitoring of , cause of pecan scab, has revealed insensitivity to fentin hydroxide and tebuconazole, but recent research indicates that the insensitivity to fentin hydroxide is not stable. A study was undertaken to determine if a fitness cost may be responsible for this instability. In this study, experiments were conducted to evaluate fitness components and phenotypic stability of insensitivity of to fentin hydroxide and tebuconazole.

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Field trials were conducted in 2015 and 2016 in Tifton, GA to determine the effects of planting dates (24 and 27 April, 4, 11, 19, and 26 May 2015; and 11, 18, and 25 April and 2, 9, and 16 May 2016), peanut () cultivar (Georgia-06G and Georgia-12Y), and seed treatment (nontreated and treated with azoxystrobin, fludioxonil, and mefenoxam) on epidemics of late leaf spot (), plant populations, and peanut yield. Final severity and AUDPC of late leaf spot increased with later planting dates in both years. For most planting dates in 2015 and the final planting date in 2016, final leaf spot severity and AUDPC were lower for Georgia-12Y than for Georgia-06G.

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The quinone outside inhibitor (QoI) fungicides are known for their inherently high resistance risk owing to substitutions in amino acid residues 129, 137, or 143 of the cytochrome gene of phytopathogens. In , cause of pecan scab, an intron adjacent to position 143 likely reduces this risk; however, the effects of a recently discovered substitution at position 137 (G137S) are unknown. Traditional in vitro assays are not useful for determining sensitivity of isolates of to the QoI fungicides, owing to the fungitoxic effects of required alternative oxidase inhibitors.

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Peanut (Arachis hypogaea) cultivars with resistance or tolerance to Cercospora arachidicola and/or Cercosporidium personatum, the causes of early and late leaf spot, respectively, are needed for organic production in the southeastern U.S. To determine the potential of new breeding lines for use in such production systems, field experiments were conducted in Tifton, GA, in 2014 and 2015 in which nine breeding lines and two cultivars, Georgia-06G and Georgia-12Y, were grown without foliar fungicide applications.

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Sclerotium rolfsii, a destructive soil-borne fungal pathogen causes stem rot of the cultivated peanut, Arachis hypogaea. This study aimed to identify differentially expressed genes associated with peanut resistance and fungal virulence. Four peanut cultivars (A100-32, Georgia Green, GA-07W and York) with increasing resistance levels were inoculated with a virulent S.

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Article Synopsis
  • - The study focused on developing effective methods to screen peanut cultivars for resistance to the root-knot nematode (Meloidogyne arenaria) to enhance agricultural practices.
  • - Greenhouse tests showed that using high inoculum levels (over 8,000 eggs/plant) allowed researchers to differentiate resistance levels quickly, while lower levels required more time; assessing gall indices based on galled roots was effective at lower inoculum levels.
  • - The timing of inoculation significantly impacted results, as plant age influenced gall indices and nematode reproduction; proper classification of resistance levels was achieved when inoculated 10 to 30 days after planting, but not at 40 days.
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Monoconidial isolates of the pecan scab fungus, Cladosporium caryigenum, were obtained in 1993 and 1994 from one pecan orchard each in Jeff Davis and Troup counties in Georgia, counties with no previous history of exposure to demethylation-inhibiting (DMI) fungicides. Isolates were grown on potato dextrose agar (PDA) amended with propiconazole or fenbuconazole at 0,0.0001, 0.

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