Impact of reporting rapid susceptibility results in Gram negative bloodstream infections: a real world prospective study.

Purpose: Mortality and morbidity of patients with bloodstream infection (BSI) remain high despite advances in diagnostic methods and efforts to speed up reporting. This study investigated the impact of reporting rapid Minimum Inhibitory Concentration (MIC)-results in Gram negative BSIs with the ASTar system (Q-linea, Uppsala, Sweden) on the adaptation of empirically started antimicrobial therapy. We performed a real-world study during which antimicrobial susceptibility testing (AST) results were instantly reported to the treating physician in an established multidisciplinary antimicrobial stewardship setting.

Partnering with Persons Living with Bipolar Disorder to Develop an Authentic Virtual Simulation.

Nursing students, faculty, and community partners report a gap in the preparation of nurses to work collaboratively with persons living with mental health conditions in a variety of healthcare settings. Engaging the expertise from lived experience within undergraduate nursing education promotes a holistic approach to care that aligns with clients' real-world needs. This paper describes the steps we followed to create a virtual simulation in partnership with persons living with mental health conditions.

How to Partner With Persons Living With Mental Health Conditions: A Guide for Meaningful Simulation Cocreation.

Screen-based simulation is an effective educational strategy that can enhance health care students' engagement with content and critical thinking across various topics, including mental health. To create relevant and realistic simulations, best-practice guidelines recommend the involvement of experts in the development process. We collaborated with persons with lived experience and community partners to cocreate a mental health-focused screen-based simulation.

Virtual clinical simulation to teach mental health concepts: a scoping review protocol.

Objective: The objective of this review is to determine the extent of the literature on the use of virtual clinical simulation to teach health professional students about mental health.

Introduction: Graduates of health professional programs need to be prepared to provide safe and effective care for persons with a mental illness in every practice context. Clinical placements in specialty areas are difficult to obtain and cannot ensure students will have opportunities to practice specific skills.

Enhancing the effectiveness of flipped classroom in health science education: a state-of-the-art review.

Background: In a flipped classroom, students acquire knowledge before class and deepen and apply this knowledge during class. This way, lower-order learning goals are achieved before class and higher-order skills are reached during class. This study aims to provide an overview of the factors that contribute to the effectiveness of the flipped classroom and how these factors can be stimulated.

Extraction and analysis of free amino acids and 5'-nucleotides, the key contributors to the umami taste of seaweed.

Assessing the umami taste of seaweed on a chemical level can inform the use and selection of seaweed in European cuisine. Accordingly, we developed a method for the simultaneous extraction, separate clean-up and analysis of 21 free amino acids and 10 free nucleotides by reversed phase and mixed-mode HPLC respectively. Of multiple mouth emulating solvents, extracting in Milli-Q at 35 °C was found most suitable.

The biogeographic differentiation of algal microbiomes in the upper ocean from pole to pole.

Eukaryotic phytoplankton are responsible for at least 20% of annual global carbon fixation. Their diversity and activity are shaped by interactions with prokaryotes as part of complex microbiomes. Although differences in their local species diversity have been estimated, we still have a limited understanding of environmental conditions responsible for compositional differences between local species communities on a large scale from pole to pole.

Motivation and Engagement of Nursing Students in 2 Gamified Courses: A Mixed-Methods Study.

Background: Evidence suggests that gamification increases student engagement in course activities. However, student feedback about gamification in nursing contexts is needed.

Purpose: The aim of this study was to describe nursing student perceptions of how gamification impacts student motivation and engagement.

Impact of off-bottom seaweed cultivation on turbulent variation in the hydrodynamic environment: A flume experiment study with mimic and natural Saccharina latissima thalli.

The seaweed industry is growing worldwide to meet future resource needs in terms of food and fuel. In the meantime, the impact of expanding off-bottom seaweed cultivation on its environment is unclear. For example, it remains poorly understood how off-bottom seaweeds affect the local hydrodynamic environment, especially concerning turbulence that is more important for nutrient transport and availability than the mean flow velocity.

The role of seasonality in reproduction of multiannual delayed gametophytes of Saccharina latissima.

Delayed gametophytes are able to grow vegetatively for prolonged periods of time. As such, they are potentially very valuable for kelp aquaculture given their great promise in opening up novel opportunities for kelp breeding and farming. However, large-scale application would require more in-depth understanding of how to control reproduction in delayed gametophytes.

A population-based study on incidence, treatment, and survival in ampullary cancer in the Netherlands.

Introduction: Ampullary cancer is rare and as a result epidemiological data are scarce. The aim of this population-based study was to determine the trends in incidence, treatment and overall survival (OS) in patients with ampullary adenocarcinoma in the Netherlands between 1989 and 2016.

Methods: Patients diagnosed with ampullary adenocarcinoma were identified from the Netherlands Cancer Registry.

Phosphate and Nitrate Uptake Dynamics in Palmaria palmata (Rhodophyceae): Ecological and Physiological Aspects of Nutrient Availability.

Uptake dynamics of dissolved inorganic phosphate (DIP) and dissolved inorganic nitrate (DIN) in young Palmaria palmata (n = 49), cultivated in a range of DIP concentrations (0.0-6.0 µmol · L ) and nonlimiting DIN concentration (50 µmol · L ) under fully controlled laboratory conditions, were quantified in a 'pulse-and-chase' approach over 5 weeks.

How light and biomass density influence the reproduction of delayed Saccharina latissima gametophytes (Phaeophyceae).

Kelp life-cycle transitions are complex and susceptible to various (a)biotic controls. Understanding the microscopic part of the kelp's lifecycle is of key importance, as gametophytes form a critical phase influencing, among others, the distributional limits of the species. Many environmental controls have been identified that affect kelp gametogenesis, whose interactive effects can be subtle and counterintuitive.

Macroalgae Derived Fungi Have High Abilities to Degrade Algal Polymers.

Marine fungi associated with macroalgae are an ecologically important group that have a strong potential for industrial applications. In this study, twenty-two marine fungi isolated from the brown seaweed sp were examined for their abilities to produce algal and plant biomass degrading enzymes. Growth of these isolates on brown and green algal biomass revealed a good growth, but no preference for any specific algae.

Ascertainment of cancer in longitudinal research: The concordance between the Rotterdam Study and the Netherlands Cancer Registry.

Complete and accurate registration of cancer is needed to provide reliable data on cancer incidence and to investigate aetiology. Such data can be derived from national cancer registries, but also from large population-based cohort studies. Yet, the concordance and discordance between these two data sources remain unknown.

Nutrient, pigment, suspended matter and turbidity measurements in the Belgian part of the North Sea.

Through regular sampling surveys, the Flanders Marine Institute is generating long term data series for the Belgian coastal water and sand bank systems, a designated site in the Long Term Ecological Research (LTER) network. The data series is built on sampling activities initiated in 2002, but gradually upgraded and extended in the framework of the LifeWatch marine observatory and the Integrated Carbon Observation System (ICOS) participation. Nine nearshore stations are sampled monthly, with additional seasonal sampling of eight offshore stations.

Uptake kinetics and storage capacity of dissolved inorganic phosphorus and corresponding dissolved inorganic nitrate uptake in Saccharina latissima and Laminaria digitata (Phaeophyceae).

Uptake rates of dissolved inorganic phosphorus and dissolved inorganic nitrogen under unsaturated and saturated conditions were studied in young sporophytes of the seaweeds Saccharina latissima and Laminaria digitata (Phaeophyceae) using a "pulse-and-chase" assay under fully controlled laboratory conditions. In a subsequent second "pulse-and-chase" assay, internal storage capacity (ISC) was calculated based on V and the parameter for photosynthetic efficiency F /F . Sporophytes of S.

Strong population structure but no equilibrium yet: Genetic connectivity and phylogeography in the kelp (Laminariales, Phaeophyta).

Kelp aquaculture is globally developing steadily as human food source, along with other applications. One of the newer crop species is , a northern hemisphere kelp inhabiting temperate to arctic rocky shores. To protect and document its natural genetic variation at the onset of this novel aquaculture, as well as increase knowledge on its taxonomy and phylogeography, we collected new genetic data, both nuclear and mitochondrial, and combined it with previous knowledge to estimate genetic connectivity and infer colonization history.

Uptake kinetics and storage capacity of dissolved inorganic phosphorus and corresponding N:P dynamics in Ulva lactuca (Chlorophyta).

Dissolved inorganic phosphorus (DIP) is an essential macronutrient for maintaining metabolism and growth in autotrophs. Little is known about DIP uptake kinetics and internal P-storage capacity in seaweeds, such as Ulva lactuca (Chlorophyta). Ulva lactuca is a promising candidate for biofiltration purposes and mass commercial cultivation.

Release of Danger-Associated Molecular Patterns following Chemotherapy Does Not Induce Immunoparalysis in Leukemia Patients.

Chemotherapy may result in the release of danger-associated molecular patterns (DAMPs), which can cause immunoparalysis (deactivation of the immune system). We investigated DAMPs following chemotherapy and their relationship with markers of immunoparalysis in leukemia patients. In 6 patients with acute myeloid leukemia or myelodysplastic syndrome and 12 healthy subjects, DAMPs, cytokines, and markers of immunoparalysis were determined before and during the first week after chemotherapy initiation.

Mitochondrial DNA and TLR9 Signaling Is Not Involved in Mechanical Ventilation-Induced Inflammation.

Exogenous administration of mitochondrial DNA (mtDNA) causes inflammatory lung injury in a toll-like receptor (TLR) 9-dependent manner. We investigated whether mechanical ventilation results in endogenous release of mtDNA and whether TLR9 plays a role in the pulmonary inflammatory response induced by mechanical ventilation.Wild-type and TLR9/ C57bl/6 mice were ventilated with low (8 mL/kg) and high (32 mL/kg) tidal volumes for 4 hours.

Plasma levels of danger-associated molecular patterns are associated with immune suppression in trauma patients.

Purpose: Danger-associated molecular patterns (DAMPs) released of trauma could contribute to an immune suppressed state that renders patients vulnerable towards nosocomial infections. We investigated DAMP release in trauma patients, starting in the prehospital phase, and assessed its relationship with immune suppression and nosocomial infections.

Methods: Blood was obtained from 166 adult trauma patients at the trauma scene, emergency room (ER), and serially afterwards.