Background: Sequence-independent amplification of clinical specimens can lead to the identification of novel pathogens.
Objectives: To identify novel viruses in human stool specimens from patients with diarrhea and to investigate the ecology and clinical significance of such viruses.
Study Design: Nucleic acid extracted from stool specimens from patients with diarrhea with no known etiology were subjected to random PCR amplification and Roche/454 pyrosequencing.
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