Publications by authors named "Tim Habenicht"
Article Synopsis
- Methanosarcina mazei strain Gö1 is a significant organism in climate research due to its methane production, and a new Ribo-seq protocol was developed to study its protein translation under different nitrogen availability conditions.
- The study identified and confidently predicted the translation of numerous small open reading frames (ORFs), with validation provided through LC-MS and immunoblotting analyses.
- A detailed differential analysis revealed that many small ORFs are regulated by nitrogen levels, highlighting their potential physiological roles and the importance of small RNAs in nitrogen metabolism within this archaeal model.
Small proteins containing fewer than 70 amino acids, which were previously disregarded due to computational prediction and biochemical detection challenges, have gained increased attention in the scientific community in recent years. However, the number of functionally characterized small proteins, especially in archaea, is still limited. Here, by using biochemical and genetic approaches, we demonstrate a crucial role of the small protein sP36 in the nitrogen metabolism of , which modulates the ammonium transporter AmtB1 according to nitrogen availability.
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