Development of syngeneic murine cell lines for use in immunocompetent orthotopic lung cancer models.

Background: Immunocompetent animal models are required to study tumor-host interactions, immunotherapy, and immunotherapeutic combinations, however the currently available immunocompetent lung cancer models have substantial limitations. While orthotopic models potentially help fill this gap, the utility of these models has been limited by the very small number of murine lung cancer cell lines capable of forming orthotopic tumors in immunocompetent C57BL/6 hosts.

Methods: Primary lung tumors with specific genetic alterations were created in C57BL/6 background mice.